作者机构：
[Wu, Jing; Zhang, Jing; Guo, Xiaohu; Han, Bangxing; Xing, Shihai; Yao, Xiaoyan; Meng, Fei] Anhui Univ Chinese Med, Coll Pharm, Hefei 230012, Peoples R China.;[Huang, Huizhen] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Peoples R China.;[Qu, Changqing] Fuyang Normal Univ, Engn Technol Res Ctr Antiaging, Chinese Herbal Med, Fuyang 236037, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, Minneapolis, MN 55108 USA.;[Wu, Jing; Xing, Shihai] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.
通讯机构：
[Xing, Shihai] A;Anhui Univ Chinese Med, Coll Pharm, Hefei 230012, Peoples R China.;Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;Anhui Prov Key Lab Res & Dev Chinese Med, Hefei 230012, Peoples R China.
摘要：
Main conclusion The Albizia julibrissin chloroplasts have a classical chloroplast genome structure, containing 93 coding genes and 34 non-coding genes. Our research provides basic data for plant phylogenetic evolutionary studies. There is limited genomic information available for the important Chinese herb Albizia julibrissin Durazz. In this study, we constructed the chloroplast (Cp) genome of A. julibrissin. The length of the assembled Cp genome was 175,922 bp consisting of four conserved regions: a 5145 bp small single-copy (SSC) region, a 91,323 bp large single-copy (LSC) region, and two identical length-inverted repeat (IR) regions (39,725 bp). This Cp genome included 34 non-coding RNAs and 93 unique genes, the former contains 30 transfer and 4 ribosomal RNA genes. Gene annotation indicated some of the coding genes (82) in the A. julibrissin Cp genome classified in the Leguminosae family, with some to other related families (11). The results show that low GC content (36.9%) and codon bias towards A- or T-terminal codons may affect the frequency of gene codon usage. The sequence analysis identified 30 forward, 18 palindrome, and 1 reverse repeat > 30 bp length, and 149 simple sequence repeats (SSR). Fifty-five RNA editing sites in the Cp of A. julibrissin were predicted, most of which are C-to-U conversions. Analysis of the reverse repeat expansion or contraction and divergence area between several species, including A. julibrissin, was performed. The phylogenetic tree revealed that A. julibrissin was most closely related to Albizia odoratissima and Albizia bracteata, followed by Samanea saman, forming an evolutionary branch with Mimosa pudica and Leucaena trichandra. The research results are helpful for breeding and genetic improvement of A. julibrissin, and also provide valuable information for understanding the evolution of this plant.
作者机构：
[Bin, Dongmei; Deng, Jingheng; Long, Jiumei; Zhou, Dongsheng; Wang, Jing; Luo, Yuanlai] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Peoples R China.;[Huang, Yayuan; Lei, Ming] Hunan Agr Univ, Coll Resource & Environm, Hunan Engn Res Ctr Safe & High Efficient Utilizat, Changsha 410128, Peoples R China.;[Yi, Zhengji] Hengyang Normal Univ, Coll Chem & Mat Sci, Hengyang 421008, Peoples R China.
通讯机构：
[Ming Lei] H;Hunan Engineering Research Center for Safe & High-Efficient Utilization of Heavy Metal Pollution Farmland, College of Resource & Environment, Hunan Agricultural University, Changsha, 410128, PR China
摘要：
It is found that the growth of Dendrobium huoshanense was dependent on Fe3O4, while the bioavailability of plants to ordinary Fe3O4 was low on the earth. In order to improve the growth, quality and yield of D. huoshanense, we used Fe3O4 NPs (100 or 200 mg/L) that was easily absorbed by plants as nano-fertilizer to hydroponically treat seedlings of D. huoshanense for 3 weeks. Fe3O4 NPs induced not only earlier flowering and increased sugar content and photosynthesis, but also stressed to plants, increased MDA content and related antioxidant enzymes activities. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) revealed that Fe3O4 NPs caused a significant accumulation of Fe and some other nutrient elements (Mn, Co, B, Mo) in stems of D. huoshanense. Metabolomics revealed that the metabolites were reprogrammed in D. huoshanense when under Fe3O4 NPs exposure. Fe3O4 NPs inhibited antioxidant defense-related pathways, demonstrating that Fe3O4 NPs have antioxidant capacity to protect D. huoshanense from damage. As the first study associating Fe3O4 NPs with the quality of D. huoshanense, it provided vital insights into the molecular mechanisms of how D. huoshanense responds to Fe3O4 NPs, ensuring the reasonable use of Fe3O4 NPs as nano-fertilizer.
摘要：
本研究旨在对临床1份腹泻病料进行病原的分离培养并探究该病原编码的主要抗原蛋白的生物学特点。采用巢式PCR检测病料猪腺病毒3型（Porcine adenovirus type 3,PADV3）核酸并进行病毒分离培养,采用免疫过氧化物酶单层细胞染色法（IPMA）进行病毒血清学鉴定,PCR扩增fiber基因,将其ORF区克隆至pET-28a（+）载体并转化大肠杆菌BL21（DE3）感受态细胞,IPTG诱导重组fiber蛋白表达,采用SDS-PAGE和Western blotting鉴定重组蛋白,将纯化的重组蛋白免疫家兔,制备多克隆抗体并测定抗体免疫活性。结果表明,病料为PADV3核酸阳性,感染ST细胞产生典型的"葡萄串"细胞病变效应（CPE）,P5～P11代次中的PADV3核酸稳定,分离的毒株P5代为PADV3型血清学阳性,fiber基因开放阅读框（ORF）为1 296 bp （GenBank登录号:MT774498）,与PADV3毒株的核苷酸相似性最高（86.1%）,与其他毒株相似性均低于40%;原核表达的重组fiber蛋白分子质量为45.2 ku,fiber蛋白多克隆抗体与PADV3感染的细胞呈特异性的细胞核染色。本试验成功分离得到1株PADV3毒株,命名为PADV3-HY1812,所制备的fiber蛋白多克隆抗体具有良好的免疫活性。
摘要：
Artemisinin is widely used as an antimalarial drug, and the regulation of artemisinin metabolism is at the forefront of artemisinin research. A WRKY transcription factor, named as AaWRKY4, was cloned from high artemisinin-yielding Artemisia annua, which has similar expression pattern with the key enzymes in artemisinin biosynthetic pathway. AaWRKY4 was preferentially expressed in glandular secretory trichomes (GSTs) of young leaves and flower buds, but weakly expressed in other tissues. To further study the function of AaWRKY4, plant expression vector pHB-AaWRKY4 containing AaWRKY4 driven by CaMV 35S promoter was constructed and introduced into A. annua via Agrobacterium tumafeciens-mediated transformation. Expression analysis showed that the expression of AaWRKY4 was increased in transgenic plants. Four independent transgenic plants overexpressing AaWRKY4 were selected for further analysis. The expression levels of artemisinin biosynthetic pathway genes ADS, CYP71AV1, DBR2 and ALDH1 were dramatically increased in AaWRKY4-overexpressing A. annua plants. Furthermore, the artemisinin yield was increased by 35–50% in AaWRKY4-overexpressing A. annua plants. These results indicate AaWRKY4 can upregulate artemisinin content through regulating artemisinin metabolism. A WRKY transcription factor, named as AaWRKY4, was cloned from high artemisinin-yielding
Artemisia annua. AaWRKY4 was preferentially expressed in glandular secretory trichomes (GSTs)
of young leaves and flower buds. The expression levels of artemisinin biosynthetic pathway genes
ADS, CYP71AV1, DBR2 and ALDH1 were dramatically increased in AaWRKY4-overexpressing A.
annua plants. Furthermore, the artemisinin yield was increased by 35–50% in AaWRKY4-
overexpressing A. annua plants.
作者机构：
[Wang, Xiao-Fan; Li, Ya; Du, Wei] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China.;[Chen, Ye] Tianjin Arts & Crafts Vocat Coll, Dept Environm Art Design, Tianjin 300250, Peoples R China.;[Wang, Shuai] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Xiao-Fan] W;Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China.
关键词：
anatomy;3D reconstruction;Anaxagorea;angiosperms;carpel;organogenesis;vascular system
摘要：
Elucidating the origin of flowers has been a challenge in botany for a long time. One of the central questions surrounding the origin of flowers is how to interpret the carpel, especially the relationship between the phyllome part (carpel wall) and the ovule. Recently, consensus favors the carpel originating from the fusion of an ovule-bearing part and the phyllome part that subtends it. Considering the carpel is a complex organ, the accurate presentation of the anatomical structure of the carpel is necessary for resolving this question. Anaxagorea is the most basal genus in a primitive angiosperm family, Annonaceae. The conspicuous stipe at the base of each carpel makes it an ideal material for exploring the histological relationships among the receptacle, the carpel, and the ovule. In the present study, floral organogenesis and vasculature were delineated in Anaxagorea luzonensis and Anaxagorea javanica, and a three-dimensional model of the carpel vasculature was reconstructed based on serial sections. The results show that in Anaxagorea, the vasculature in the carpel branches in the form of shoots. The radiosymmetrical vasculature pattern is repeatedly presented in the receptacle, the carpel, and the funiculus of the ovule. This provides anatomical evidence of the composite origin of the carpel.</p>
