摘要:
The Hymenasplenium obliquissimum group contains the widespread H. obliquissimum and several geographically restricted species, including H. retusulum, H. wuliangshanense, H. latidens, H. changputungense, H. quercicola, H. szechuanense, H. furfuraceum, H. adiantifrons, and H. filipes. However, the taxonomy of this group is still unclear and needs to be revised because some entities were treated infraspecifically or as synonyms and the validation of some species still needs to be assessed. To formulate a natural classification and investigate the relationships in this group, we collected and studied specimens of species related to the H. obliquissimum group and obtained specimens of species described by Ching at their locus classicus in southwestern China. An integrative taxonomic approach was taken to delimit species in the group using cytological, morphological, and DNA sequence data. Specifically, in the phylogenetic analyses, the H. obliquissimum group was recovered as a monophyletic group, comprising five principal chloroplast lineages. Based on our inferences, we provided taxonomic implications of chloroplast lineages discovered in this study and suggested possible reticulate evolution in the H. obliquissimum group which was interpreted by the incongruence of chloroplast and nuclear phylogenies. Further studies to strengthen the taxonomic of taxa especially those with the co-existence of different ploidy levels are still warranted.
作者机构:
[Zhang Hao; Chen Wei; Wang Gang; He Yufeng; Zhao Jianxin; Jin Xing] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China.;[Zhang Hao; Chen Wei; Wang Gang; He Yufeng; Zhao Jianxin; Jin Xing] Jiangnan Univ, Schl Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China.;[Zhou Yonghua] Jiangsu Inst Parasit Dis, Jiangsu Prov Key Lab Parasite & Vector Control Te, Key Lab Natl Hlth & Family Plann Commis, Parasit Dis Control & Prevent, Wuxi 214064, Jiangsu, Peoples R China.;[Chen Xiaohua] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Peoples R China.;[Lee Yuan-Kun] Natl Univ Singapore, Dept Microbiol & Immunol Natl, Singapore 117597, Singapore.
通讯机构:
[Wang, Gang] S;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.;School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.;International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi, 214122, P. R. China.;(Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, 225004, P. R. China.
摘要:
Campylobacter jejuni is the major micro-bacillary pathogen responsible for human coloenteritis. Lactic acid bacteria (LAB) have been shown to protect against Campylobacter infection. However, LAB with a good ability to inhibit the growth of C. jejuni in vitro are less effective in animals and animal models, and have the disadvantages of high cost, a long cycle, cumbersome operation and insignificant immune response indicators. Caenorhabditis elegans is increasingly used to screen probiotics for their anti-pathogenic properties. However, no research on the use of C. elegans to screen for probiotic candidates antagonistic to C. jejuni has been conducted to date. This study established a lifespan model of C. elegans, enabling the preselection of LAB to counter C. jejuni infection. A potential protective mechanism of LAB was identified. Some distinct LAB species offered a high level of protection to C. elegans against C. jejuni. The LAB strains with a high protection rate reduced the load of C. jejuni in C. elegans. The transcription of antibacterial peptide genes, MAPK and Daf-16 signalling pathway-related genes was elevated using the LAB isolates with a high protection rate. The reliability of the lifespan model of C. elegans was verified using mice and chickens infected with C. jejuni. The results showed that different LAB had different abilities to protect C. elegans against C. jejuni. C. elegans provides a reliable model for researchers to screen for LAB that are antagonistic to C. jejuni on a large scale.
期刊:
Mitochondrial DNA Part B-Resources,2021年6(8):2174-2176 ISSN:2380-2359
通讯作者:
Shihai Xing
作者机构:
[Wu, Jing; Zhang, Jing; Wu, Liping; Guo, Xiaohu; Xing, Shihai; Yao, Xiaoyan; Meng, Fei] Anhui Univ Chinese Med, Coll Pharm, Hefei 230012, Peoples R China.;[Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang, Peoples R China.;[Wu, Jing; Xing, Shihai] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, 1 Qianjiang Rd, Hefei 230012, Peoples R China.;[Xing, Shihai] Anhui Prov Key Lab Res & Dev Chinese Med, Hefei, Peoples R China.
通讯机构:
[Shihai Xing] C;College of Pharmacy, Anhui University of Chinese Medicine, Hefei, China<&wdkj&>Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, China<&wdkj&>Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei, China
关键词:
Epilobium hirsutum;Onagraceae;chloroplast genome;phylogenetic tree
摘要:
The complete chloroplast genome sequence of Epilobium hirsutum L. is presented here. It is 161,111 bp in length and divides into four distinct regions: a small single-copy region (SSC) of 17,310 bp, a large single-copy region (LSC) of 89,117 bp, and a pair of inverted repeat (IR) regions of 27,342 bp. The chloroplast genome of E. hirsutum includes a total of 125 genes, consisting of 31 tRNA genes, 8 rRNA genes, and 86 protein-coding genes. A phylogenetic tree was generated to evaluate the evolutionary relationship between E. hirsutum and relevant species. The chloroplast genome sequencing and phylogenetic analysis offer genetic background for conservation and phylogenetic studied of this species.
通讯机构:
[Ma, Aimin] C;College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.;Key Laboratory of Agro-Microbial Resources and Utilization, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China.
关键词:
Dimorphism;G protein α subunit;Gene function;Morphological change;Tremella fuciformis
摘要:
Tremella fuciformis is a dimorphic fungus which can undertake the reversible transition between yeast and pseudohypha forms. G protein α subunit (Gα) carries different signals to regulate a variety of biological processes in eukaryotes, including fungal dimorphism. In this study, a novel Gα subunit encoded gene, TrGpa1, was firstly cloned from T. fuciformis. The TrGpa1 open reading frame has 1059 nucleotides, and encodes a protein which belongs to the group I of Gαi superfamily. Furthermore, the role of TrGpa1 in the T. fuciformis dimorphism was analysed by gene overexpression and knockdown. Stable integration of the target gene into the genome was confirmed by PCR and Southern blot hybridization. Transformants with the highest and lowest TrGpa1 expression levels were selected via quantitative real-time PCR analysis and Western blot. Each transformant was compared with the wild-type strain about the morphological change under different environmental factors, including pH values, temperature, cultivation time, inoculum size, and quorum-sensing molecules (farnesol and tyrosol). Comparing with the wild-type strain, the overexpression transformant always had higher ratios of pseudohyphae, while the knockdown transformant had less proportions of pseudohyphae. Therefore, the TrGpa1 is involved in the dimorphism of T. fuciformis and plays a positive role in promoting pseudohyphal growth.
期刊:
Entomologia Experimentalis et Applicata,2021年169(11):1039-1048 ISSN:0013-8703
通讯作者:
Jiu-Mei Long
作者机构:
[Teng, Tao; Liu, Jian-Hui; Zhou, Dong-Sheng; Long, Jiu-Mei] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang, Peoples R China.
通讯机构:
[Jiu-Mei Long] H;Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences and Environment, Hengyang Normal University, Hengyang, China
作者:
Su, Xinglong;Liu, Yingying;Han, Lu;Wang, Zhaojian;Cao, Mengyang;...
期刊:
Scientific Reports,2021年11(1) ISSN:2045-2322
通讯作者:
Shihai Xing<&wdkj&>Daiyin Peng
作者机构:
[Wu, Liping; Guo, Xiaohu; Xing, Shihai; Su, Xinglong; Cao, Mengyang; Wang, Zhaojian; Yu, Nianjun; Peng, Daiyin; Han, Lu; Meng, Fei; Gui, Shuangying] Anhui Univ Chinese Med, Sch Pharm, Hefei 230012, Peoples R China.;[Xing, Shihai; Su, Xinglong; Cao, Mengyang; Wang, Zhaojian; Peng, Daiyin] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;[Liu, Yingying] Anhui Univ Chinese Med, Coll Humanities & Int Educ Exchange, Hefei 230012, Peoples R China.;[Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Hunan, Peoples R China.;[Gui, Shuangying] Anhui Univ Chinese Med, Anhui Prov Key Lab Pharmaceut Preparat Technol &, Hefei 230012, Peoples R China.
通讯机构:
[Shihai Xing; Daiyin Peng] S;School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China<&wdkj&>Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, China<&wdkj&>Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Hefei, China<&wdkj&>School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China<&wdkj&>Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, China<&wdkj&>Anhui Province Key Laboratory of Research and Development of Chinese Medicine, Hefei, China
摘要:
Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally. Studies have shown beta -Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus. An L-9(3(4)) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS+NAA 1.0 mg/L+6-BA 0.5 mg/L to obtain callus for experimental use. The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly. Platycodin D and total polysaccharide content of calli was found higher than that of leaves. While, platycoside E and total polysaccharide content of calli was found lower than that of leaves. Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of beta -Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus. Totally, a candidate gene encoding enzyme involved in converting platycoside E to platycodin D, and putative genes involved in polysaccharide synthesis in P. grandiflorus had been identified. This study will help uncover the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus.