摘要:
Pseudomonas aeruginosa is a conditional Gram-negative pathogen that produces extracellular virulence factors that can lead to bloodstream invasion, severely harm tissues, and disseminate bacteria, ultimately leading to various diseases. In this study, lactic acid bacteria (LAB) with strong antagonistic ability against P. aeruginosa were screened, and the regulatory mechanism of LAB against P. aeruginosa was evaluated. The results showed that the three selected LAB strains had strong inhibition ability on the growth, biofilm formation, and pyocyanin expression of P. aeruginosa and a promoting effect on the expression of autoinducer-2. Among them, Lactipantibacillus plantarum (Lp. plantarum) LPyang is capable of affecting the metabolic processes of P. aeruginosa by influencing metabolic substances, such as LysoPC, oxidized glutathione, betaine, etc. These results indicate that LPyang reduces the infectivity of P. aeruginosa through inhibition of its growth, biofilm formation, pyocyanin expression, and regulation of its metabolome. This study provides new insights into the antagonistic activity of Lp. plantarum LPyang against P. aeruginosa.
期刊:
Zeitschrift fur Kristallographie - New Crystal Structures,2023年238(2):367-369 ISSN:1433-7266
作者机构:
[Xiao, Zhenxiu] Hengyang Normal Univ, Nanyue Coll, Hengyang 421008, Hunan, Peoples R China.;[Wang, Tiantian; Deng, Xin] Hengyang Normal Univ, Sch Chem & Mat Sci, Hengyang 421008, Hunan, Peoples R China.;[Jiang, Wujiu; Tan, Yuxing] Normal Univ, Key Lab Met Organ New Mat Coll Hunan Prov Hengyang, Sch Chem & Mat Sci, Key Lab Funct Met Organ Cpds Hunan Prov, Hengyang 421008, Hunan, Peoples R China.
摘要:
C24H30N4O4, monoclinic, C2/c (no. 15), a = 21.170(3) angstrom, b = 13.2938(17) angstrom, c = 9.7133(12) angstrom, beta = 114.423(2)degrees, V = 2489.0(5) angstrom(3), Z = 4, R-gt(F) = 0.0473, wR(ref)(F-2) = 0.1424, T = 296(2) K.
期刊:
Journal of Inorganic Biochemistry,2022年232:111808 ISSN:0162-0134
通讯作者:
Wujiu Jiang<&wdkj&>Yuxing Tan
作者机构:
[Jiang, Wujiu; Tan, Yuxing; Qin, Qiqi; Xiao, Xiyuan] Hengyang Normal Univ, Nanyue Coll,Coll Chem & Mat Sci, Key Lab Funct Met Organ Cpds Hunan Prov,Key Lab O, Coll Hunan Prov,Hunan Prov Engn Res Ctr Monitorin, Hengyang 421008, Hunan, Peoples R China.
通讯机构:
[Wujiu Jiang; Yuxing Tan] K;Key Laboratory of Functional Metal-Organic Compounds of Hunan Province, Key Laboratory of Organometallic New Materials, College of Hunan Province, Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of XiangJiang River, College of Chemistry and Materials Science, Nanyue College, Hengyang Normal University, Hengyang, Hunan 421008, PR China<&wdkj&>Key Laboratory of Functional Metal-Organic Compounds of Hunan Province, Key Laboratory of Organometallic New Materials, College of Hunan Province, Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of XiangJiang River, College of Chemistry and Materials Science, Nanyue College, Hengyang Normal University, Hengyang, Hunan 421008, PR China
摘要:
Eight new diorganotin(IV) complexes (1a-2d), namely {[X-C6H4(O)C=N-N=C(Me)COO]R2Sn(CH3OH)}(n) (1a, 2a), {[X-C6H4(O)C=N-N=C(Me)COO]R2Sn(CH3OH)}(2) (1b, 1c, 1d, 2b), and {[X-C6H4(O)C=N-N=C(Me)COO]R2Sn}(2) (2c, 2d) (X = H-, p-Me-, p-OH-, p-NO2-; R = o-Cl-C6H4CH2- or o-Me-C6H4CH2-), have been synthesized by microwave "one-pot" reaction with arylformylhydrazine, pyruvic acid, and the corresponding R2SnCl2. All the complexes have been characterized by FT-IR (Fourier transform infrared spectroscopy), multinuclear NMR (H-1, C-13, and Sn-119 nuclear magnetic resonance spectroscopies), HRMS (high-resolution mass spectroscopy) and single-crystal X-ray structural analysis. The antiproliferative activity of all complexes was tested against the cancer cell lines NCI-H460, MCF-7 and HepG2. The diorganotin complex 1c has been shown to be more potent antitumor agents against HepG2 than other complexes and cisplatin. Flow cytometry analysis observation demonstrated that complex 1c mediated cell apoptosis of HepG2 cells and arrested cell cycle in the S phase. The single cell gel electrophoreses assay results show that the 1c induce DNA damage. The DNA binding activities of the 1c were studied by UV-visible absorption spectrometry, fluorescence competitive, circular dichroism measurements, and molecular docking, results shown 1c can be well embedded in the groove and cleave DNA.