Design of data structure is valuable to the concrete realization of DNA computer. Therefore, a method of designing a binary tree based on sequential storage model in DNA computer was proposed, which utilized the biological characteristics of DNA molecules and restriction endonucleases to complete sequential storage structure and basic operations of the binary tree. All the biological technology mentioned here could be practically implemented in the laboratory. To prove the feasibility of this method, an actual binary tree with detailed nucleotide encodings and an instance of algorithm were giv...