A polythiophene functionalized with methylene blue (PMT-MB) was synthesized and used as an indicator for electrochemical oligonucleotides (ODNs) hybridization detection. After hybridization with complementary ODNs, the current signal of PMT-MB increased, which illustrated that PMT-MB can effectively recognize complementary ODN targets as an indicator. Compared to MB, PMT-MB showed much better resistance to the concentration change of buffer solution. In all buffer solutions tested, the hybridization always resulted in the increased current signal of PMT-MB due to the electrostatic interaction....
