Artemisinin is widely used as an antimalarial drug, and the regulation of artemisinin metabolism is at the forefront of artemisinin research. A WRKY transcription factor, named as AaWRKY4, was cloned from high artemisinin-yielding Artemisia annua, which has similar expression pattern with the key enzymes in artemisinin biosynthetic pathway. AaWRKY4 was preferentially expressed in glandular secretory trichomes (GSTs) of young leaves and flower buds, but weakly expressed in other tissues. To further study the function of AaWRKY4, plant expression...