Abstract A highly sensitive method for the spectrophotometric determination of molybdenum with o-hydroxynaphthyl fluorone and DDMBA has been investigated. The molar absorptivity of the com-plex is 1.47×10~5 at 550nm. The molar ratio of Mo(Ⅵ) to β-HNF in the complex was determined to be 1∶2.Beer's law is obeyed in the range of 0 to 5μgMo/25ml.This method has good selectivity,many other metal ions don't inrerfere with the determination.A new method for the spectrophoto-metric determination of molybdenum has been used to analyse several...