An acifluorfen-imprinted polymer (P1) was prepared by the combined use of bismethacryloyl-β-cyclodextrin (BMA-β-CD) and 4-vinylpyridine (4-VP) as functional monomers. Compared with the molecularly imprinted polymers (MIPs) using only BMA-β-CD or 4-VP as a functional monomer, P2 and P3, respectively, P1 showed higher binding capacity (Cp) and imprinting factor (IF) for acifluorfen in aqueous media. Scatchard plot analysis revealed that two classes of binding sites were formed in the imprinted polymer P1 with dissociation constants of 0.435 an...