期刊论文

中文

食品与机械

1003-5788

2021

37

10

6-11,149

10.13652/j.issn.1003-5788.2021.10.002

湖南省自然科学基金（编号：2019JJ50014）； 国家自然基金青年基金（编号：31601444）； 衡阳师范学院大学生创新创业训练计划项目（编号：cxcy1934）;衡阳师范学院大学生课外学术科技作品竞赛（编号：校科字[2019]2号）；

本校为其他机构

目的:以铜绿假单胞菌（Pseudomonas aeruginosa, PA）为指示菌,基于群体感应系统筛选拮抗PA作用的乳酸菌（Lactic acid bacteria,LAB）并探讨拮抗机制。方法:通过牛津杯法筛选抑制PA生长的LAB,测定LAB的AI-2表达量,LAB抑制PA生物膜形成力、绿脓菌素表达量及LAB无菌上清液与PA共培养时AI-2信号分子的表达量,并利用皮尔森相关性分析探讨LAB拮抗PA机制。结果:格氏乳杆菌LGchen和唾液乳杆菌S-5-6的整体抗菌效果比较强,抑菌圈达到20 mm以上,AI-2相对荧光值达到1.2以上,抑制PA的绿脓菌素表达率达50%以上,抑制PA生物膜形成率达70%左右。LAB抑制PA生长的能力与LAB的表达AI-2的能力、抑制PA的生物膜形成能...

Objective:In this study,Pseudomonas aeruginosa(PA)was used as the indicator bacteria,and based on the quorum sensing system,the lactic acid bacteria(LAB)that antagonized the effect of PA were screened and the antagonistic mechanism was preliminarily discussed.Methods:The mechanism of LAB antagonizing PA was explored by the tests of Oxford Cup Method screening LAB to inhibit the growth of PA,AI-2 expression of LAB,biofilm formation ability of PA,pyocyanin expression and the AI-2 expression of PA with LAB sterile supernatants,and the analysis of Pearson correlation.Results:The results showed tha...