期刊论文

Jiang Jian-Hui

中文

分析化学

0253-3820

2007

35

10

1435-1438

10.1016/S1872-2040(07)60086-3

Received 1 February 2007; accepted 25 May 2007 * Corresponding author. Email: jianhuijiang@hnu.cn This work was supported by the National Basic Research Program of China (No. 20205005). Copyright © 2007, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Published by Elsevier Limited. All rights reserved.

本校为其他机构

用镀铂碳糊电极电聚酪胺和过氧化聚吡咯固定葡萄糖氧化酶(GOD)制成电流型葡萄糖传感器.铂黑沉积层的微孔结构使电极具有较大的有效表面积,增加了固定的酶量,电极灵敏度提高.通过聚合酪胺(PTY)和过氧化聚吡咯(OPPY),可提高酶电极的稳定性和抗干扰能力.该电极具有响应时间短(＜10 s)、响应范围宽(0.01～18 mmol/L)、灵敏度高(检出限为5 μmol/L)、稳定性好等优点,电极经过60 d、约60次反复测试后,响应电流下降至最初值的70%,并具有抗果糖、尿酸和抗坏血酸干扰的特点.

An amperometric glucose biosensor has been developed based on immobilization of glucose oxidase (GOD) in electrochemically polymerized polytyramine (PTY) and overoxidised polypyrrole (OPPY) on platinized carbon paste electrode (PCPE). By providing a large apparent surface area with the micropores of the electrodeposited platinum particles on the PCPE, GOD was added, and the sensor was more sensitive. Owing to the polymerized polytyramine film and overoxidised polypyrrole film on the PCPE, the sensor was more anti-interferential and more stable....