期刊论文

Liu Meng-Qin

中文

分析化学

0253-3820

2010

38

2

258-262

10.3724/SP.J.1096.2010.00258

国家自然科学基金项目（No.20205005）； 新世纪优秀人才支持计划基金（No.NCET-04-0768）； 湖南省重点学科建设项目； 湖南省教育厅科研计划项目（No.07C162）资助；

本校为第一且通讯机构

将酪胺应用于酶联免疫分析,建立了一种新的高灵敏伏安型免疫传感器.利用纳米金的静电吸咐和己二硫醇、巯基乙胺的自组装,将羊抗人IgG抗体固定到金电极表面上,以辣根过氧化物酶标记羊抗人IgG抗体为酶标抗体,以生物素化酪胺为酶底物,利用催化酪胺沉积反应,在传感界面沉积大量生物素,使原始信号得到几何级数的放大.结果表明,通过生物素化酪胺催化放大后,制得的免疫传感器对H_2O_2的催化能力增大近20倍,检测hIgG在1.5 μg/L～22 mg/L范围内有良好的线性关系,检出限为0.1 μg/L.用于实际试样的回收率的测定, 结果良好.

A novel and highly sensitive voltammetric enzyme-linked immunosensor was developed based on tyramine oxidation deposition. It was shown that gold nano-particles (colloid Au) could be used as a platform to immobilize antibodies by adsorption. By a sandwich immunossary format with goat-anti-human IgG labled Horseradish peroxidase (HRP) as the second antibody and catalytic amplification by biotin-tyramine, the immunosensor's catalytic ability to hydrogen peroxide increased nearly 20 times, the sensor exhibitd a linear response to human IgG in the concentration range from 1.5 mu g/L - 22 mg/L, and...