期刊论文

Study on tissue culture and rapid propagation of Begonia mannii

中文

广西农业生物科学

Journal of Guangxi Agricultural and Biological Science

1009-606X

2006

13

03

265-268

金华职业技术学院校级科研基金项目（20041120）.

本校为其他机构

采用盆栽玫瑰海棠的叶片作外植体进行组织培养试验。结果表明:用0.1%HgC l2对叶片表面灭菌10 m in的死亡率和感染率较低,接种于3/4 M S+6-BA 2.5 m g/L+NAA 1.0 m g/L+琼脂7.8 g/L+蔗糖25g/L的培养基上诱导出新生芽,在3/4 M S+6-BA 1.0 m g/L+NAA 0.2 m g/L+琼脂7.8 g/L+蔗糖25 g/L的培养基上,增殖系数可达6~8;在1/2M S+KT 2.0 m g/L+NAA 0.3 m g/L+琼脂7.8 g/L+蔗糖25 g/L的培养基上培养25 d左右,可形成6~9条粗壮的根和2.0~3.0 cm高的完整植株,试管小苗带培养基移栽到泥炭+泥沙+锯屑(1∶1∶1)的基质上,成活率达95%。

Tissues cultures were carried out using the blade of Begonia rnannii in pot as explants. Results showed that explants of blade＇s which their surfaces treated by 0. 1 % HgCl2 for 10 min could obtain low rate of dead and infection, and they could be induced to regenerate plant when they were inoculated in culture medium of 3/4 MS＋6-BA 2.5 mg/L＋NAA 1.0 mg/L＋Agar 7.8 g/L＋Sucrose 25 g/L. The regenerated plant of B. rnannii cultured in 3/4 MS＋6-BA 1.0 mg/L＋NAA 0.2 mg/l.＋ Agar7. 8 g/L ＋ Sucrose25 g/Lhadthepropagation coefficient of 6 ～ 8. An...