期刊论文

Laboratory Diagnosis on a Case of Infection with Wild Type Pseudorabies Virus

中文

中国动物检疫

1005-944X

2018

35

9

77-80

10.3969/j.issn.1005-944X.2018.09.020

国家自然基金青年基金（31101837） 衡阳师范学院***专项（16D20） 湖南省教育厅优秀青年项目（17B041）

本校为第一机构

为确诊衡阳市某猪场育肥猪发生的疑似猪伪狂犬病(PR)疫情,采集发病猪脾脏,运用PCR方法进行猪伪狂犬病病毒(PRV)核酸检测,利用非洲绿猴肾细胞(Vero)进行病毒分离培养,采用免疫过氧化物酶单层细胞染色法(IPMA)进行病毒血清学鉴定;用分离培养物进行家兔感染试验,观察临床症状和病例剖检变化,采用PCR检测各器官中的病毒分布情况.结果显示:病料接种Vero细胞培养3 d后,出现明显的细胞病变效应(CPE);分离培养的病毒与PRV阳性血清呈阳性反应;家兔感染后出现奇痒等神经症状并死亡,其肝、脾组织均为PRV gE核酸检测阳性.试验结果证实,分离到的病毒为PRV野毒,从而确诊了该疑似疫情.

In order to identify the suspected porcine pseudorabies（PR）epidemic in fattening pigs in a farm of Hengyang City,the spleens of infected pigs were collected and porcine pseudorabies virus（PRV）nucleic acids were detected by PCR method. Then,virus isolation and serological identification were carried out by Vero cells and immunoperoxidase mono-layer assay（IPMA）. The rabbits were infected with the isolated culture and clinical symptoms and necropsy changes were observed. Finally,the virus distribution situation in different organs was determ...