期刊论文

Isolation and Identifi cation of a Strain of Type I Canine Adenovirus

中文

中国动物检疫

1005-944X

2019

36

2

82-87

10.3969/j.issn.1005-944X.2019.02.021

201810546002:国家级大学生创新创业训练计划项目 201710546001:国家级大学生创新创业训练计划项目 17B041:湖南省教育厅优秀青年项目 ZXXQ201701:校企合作项目 18H03:校企合作项目 16D20:***专项

本校为第一机构

为给I型犬腺病毒（CAV-I）后续研究提供基础材料,采集疑似犬传染性肝炎患犬粪便,用PCR方法进行CAV-I核酸检测,并运用犬肾传代细胞（MDCK）进行病毒分离培养,采用PCR和免疫过氧化物酶单层细胞染色法（IPMA）检测病毒在细胞内的繁殖状态。结果显示:患犬粪便样品PCR检测为CAV-I核酸阳性,病料接种MDCK细胞培养24 h后出现明显的"葡萄串样"细胞病变效应（CPE）;培养物血清学鉴定为CAV抗原阳性,不同代次病毒培养物均为CAV-I核酸强阳性;第9代病毒核酸序列与Gen Bank中CAV-I毒株核苷酸相似性在99.0%以上,与CAV-Ⅱ相似性仅为65.3%;该病毒与CAV-I毒株处于同一遗传分支;病毒接种MDCK后8 h内均无抗原检出,12 ...

In order to isolate and identify type I canine adenovirus(CAV-I)so as to provide basic materials for subsequent researches,feces of dogs suspected of infecting CAV-I were sampled,and nucleic acid test for CAV-I was conducted with PCR,then madin-darby canine kidney cells(MDCK)were adopted for virus isolation and cultivation.What's more,PCR and immunoperoxidase mono-layer assay(IPMA)were applied to detect the proliferation of viruses in the cells.The results of PCR detection were positive for CAV-I nucleic acid.With 24 hours of cultivation for samples with MDCK,obvious botryoidal-shaped cytopath...