[ Objective ] The aim was to study the sleep deprivation on sex behavior and sperm quality of male mice. [ Method ] The mice in the treated groups (36 h) were sleep deprived using gentle handling method. Sex behavior, sperm motility and the sperm abnormality were ex- amined in treated and control group. [ Result] There is extremely significant difference between 36 h sleep deprivation group and the control group in capture times and capture incubation period ( both P 〈 O. O1 ) ; and there are significant difference in treated group compare...