The two-dimensional (2D) molecular imprinting approach has attracted extensive research interest in recent years due to its potential advantages such as simple construction, fast template removal and rapid mass transfer. In this study, a new 2D imprinting approach based on the combination of mesoporous silica materials and molecular imprinting technology is reported. 2D molecularly imprinted materials (MIMs) for cholesterol were prepared by using cholesterol as the template, azide modified β-cyclodextrin (azide-β-CD) as the functional monomer and alkynyl-modified SBA-15 (alkyne-SBA-1...