期刊论文

中文

纤维素科学与技术

1004-8405

2018

26

2

46-52

10.16561/j.cnki.xws.2018.02.03

2015JJ2019:湖南省自然科学基金 2015KJ25:衡阳市科技局一般项目 14A04:衡阳师范学院青年项目

本校为第一机构

以滤纸条为唯一碳源培养基对富含纤维素的土样微生物进行富集培养,再用刚果红纤维素琼脂培养基筛选降解效果好的菌株,根据菌落形态及显微结构进行鉴定,并测定其发酵液的FPAase酶活和对稻草秸秆的降解情况。通过刚果红纤维素琼脂培养基筛选得到的最具纤维素分解能力的真菌经形态鉴定为冬克青霉（Penicillium donkii）,该菌在含有羧甲基纤维素钠的液体培养基中28℃,180 r/min培养,在第8d酶活力达到最大为15.0 U/m L。接种菌株21d的稻草纤维素含量由接种前的30.30%减少至26.36%。青霉1-4为冬克青霉,对纤维素有一定的降解能力,可以加速对稻草秸秆中纤维素的降解。

The strains were cultured in the medium with filter strips as the sole carbon source and isolated with PDA medium from soil samples with rich cellulose in Hengyang. Then cellulose-degrading fungi were selected in Congo red cellulose agar. One strain (numbered 1-4) was screened by the ratio colony diameter to diameter of transparent circle and colony diameter. The strain was identified by the morphological characteristics on the identification culture medium (CYA) with three inoculation method and inserted cover glass. Then the strain was inocul...