期刊论文

A Daedalea modesta strain with nematicidal activity and its role in killing nematodes

中文

菌物学报

1672-6472

2016

35

10

1218-1225

10.13346/j.mycosystema.150206

广东省计划项目（2005B20801013,2007B020709008） 国家农业行业项目（nyhyzx07-050-4） “十一五”国家科技支撑计划（2006BAD08A08） 教育部博士点基金（20050564009） 湖南省自然科学基金（2015JJ2019）

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为了研究野生担子菌对线虫的作用效果,从腐木桩上采集担子菌,应用常规组织分离法获得纯培养菌丝,以全齿复活线虫作靶标,初步筛选到1株对线虫有高活性的菌株,应用形态学方法和分子生物学方法进行了种的鉴定,测定了其对全齿复活线虫Panagrellus redivivus、松材线虫Bursaphelenchus xylophilus、爪哇根结线虫Meloidogyne javanica的杀线虫活性,并测定了其在不同条件下的发酵液对全齿复活线虫、松材线虫和爪哇根结线虫二龄幼虫的作用效果。经鉴定该菌株为谦逊迷孔菌Daedalea modesta,其菌丝在平板上能够迅速杀死线虫,平板菌落上接种线虫48h,菌丝对全齿复活线虫、松材线虫和爪哇根结线虫的致死率分别...

A basidiomycete strain destructive to nematodes was screened out by using routine tissue-separation method and Panagrellus redivivus as a target. The strain was identified as Daedalea modesta. The effect of mycelia of the strain on attacking P. redivivus, Bursaphelenchus xylophilus and Meloidogyne javanica were tested, and the effect of fermentation filtrates of the mycelia cultured in different conditions on P. redivivus, B. xylophilus and second instar larvae of M. javanica were also determinated. The mortality of P. redivivus, B. xylophilus ...