期刊论文

Two basidiomycetes destructive to nematode Bursaphelenchus xylophilus

中文

菌物学报

1672-6472

2020

39

2

457-463

10.13346/j.mycosystema.190133

Supported by Natural Science Foundation of Hunan Province (2015JJ2019). Corresponding author. E-mail: yzl_2002@163.com Received: 2019-04-19, accepted: 2019-10-26

本校为第一机构

为了筛选有效的杀松材线虫野生担子菌菌株,以期用于开发安全高效杀松材线虫生防制剂,从野外采集担子菌子实体,采用常规组织分离法获得纯培养菌株303株,以全齿复活线虫Panagrellus redivivus作靶标进行皿内菌丝杀线虫活性测定,获得2株高活性的菌株BSN003和BSN034,结合形态学和ITS序列分析,将菌株BSN003鉴定为Neonothopanus nambi,菌株BSN034鉴定为棕榈微皮伞Marasmiellus palmivorus;两菌株的菌丝在平板上对松材线虫Bursaphelenchus xylophilus均有较强的杀线虫能力,在长有菌落的CMA平板上接种松材线虫48h,菌株BSN003处理线虫的死亡率为100%,菌株BSN034处理线虫的捕食率为93.6%。本研究表明N.nam...

Three hundred and three fungal strains were isolated from fruitbodies of wild basidiomycetes and their toxicity against nematode Panagrellus redivivus were evaluated in Petri dish. Two strains showed high toxic to P. redivivus were identified based on morphology of fruitbody and analysis of ITS sequence. Strain BSN003 was identified as Neonothopanus nambi and the strain BSN034 was identified as Marasmiellus palmivorus. These two fungi also showed high toxicity to pine wood nematode Bursaphelenchus xylophilus with mortality of 100% by treatment ...