期刊论文

Isolation of facultative anaerobic entophytic bacterial companioned Ca Las infected citrus tissues and evaluation of dominant bacterial populations

中文

微生物学通报

Microbiology

0253-2654

2011

25

9

1362-1370

国家自然科学基金项目(No.30971875)； 公益性行业(农业)科研专项(No.201003067)； 校企科研合作项目(No.200706201312)；

本校为其他机构

以定向分离培养和基于16S rDNA的PCR-DGGE (Denaturing gradient gel electrophoresis,DGGE)方法,分析感黄龙病柑橘与健康柑橘植株不同部位的内生细菌多样性,分离柑橘组织共获得19株可培养的兼性厌氧型内生细菌,经形态、生理生化结合16S rDNA分子方法鉴定其隶属于12个属,其中短小杆菌属Curtobacterium sp.(IF:29.07％)、芽孢杆菌属Bacillus sp.(IF:23.12％)和微杆菌属Microbacterium sp.(IF:21.09％)为罹病植株的优势菌群,芽孢杆菌属Bacillus sp.(IF:21.03％)、动性球菌属Planococcus sp.(IF:20.69％)和假单胞菌属Pseudomonas sp.(IF:17.44％)为无症健株的优势茵群.对DGGE方法得到的50务16S rDN...

To analysis the entophytic bacterial diversity of citrus and find the companion bacteria populations associated with Huanglongbing pathogen-infected and healthy citrus plant tissues for decipher the co-cultivation of HLB pathogen,we selected varied parts of citrus tissues collected from different locations of citrus planted area.The facultative anaerobic entophytic bacteria were isolated and purified based on bacterial morphology,physiology,biochemistry characteristics and the molecular method of PCR-DGGE（Denaturing gradient gel electrophoresi...