Glutamine dependence is a prominent feature of cancer metabolism and is essential for the growth of CT26 cells. The photobiomodulation of glutamine‐dependent CT26 cells inhibits cell apoptosis without increasing cell proliferation by regulating GLUT1, MEK/ERK, and PI3K/AKT/mTOR signals. The proliferation and apoptosis of CT26 cells with glutamine addiction are jointly completed by multiple signaling pathways. Abstract We investigated the mechanism of action of photobiomodulation (PBM) with light‐emitting diode (led) 640 nm of glutamine‐depe...