期刊论文

Sun, Weifeng

英文

Bioprocess and Biosystems Engineering

1615-7591

2020

43

4

701-710

10.1007/s00449-019-02268-6

This work was supported by the National Natural Science Foundation of China (31601418, 31601444 and 21506172), the School level project of Hengyang Normal University (18D14), the Applied Basic Research Programs of Science and Technology Department of Sichuan Province (2019YJ0390), the Spring Sunshine Plan of the Ministry of Education of China (Z2015121), the Key Project of Xihua University of China (z1420524 and z1420525), the Department of Education of Sichuan Province of China (15205445), the Key Laboratory program of Food and Biotechnology of Xihua University of China (szjj2015-013 and szjj2015-014). Undergraduate Training Program for Innovation (201510623003 and 201510623053).

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Currently, some cases about the expression of flavor peptides with microorganisms were reported owing to the obvious advantages of biological expression over traditional methods. However, beefy meaty peptide (BMP), the focus of umami peptides, has neither been concerned in its safe expression nor its overproduction in fermenter. In this study, multi-copy BMP (8BMP) was successfully auto-inducibly expressed and efficiently produced in Bacillus subtilis 168. First, 8BMP was successfully auto-inducibly expressed with srfA promoter in B. subtilis 168. Further, the efficient production of 8BMP was ...