Simultaneous ultrasonic/microwave assisted hydrodistillation extraction (UMHE) was first employed to extract essential oil. Meanwhile, the effects of UMHE, hydrodistillation extraction (HE), microwave assisted hydrodistillation extraction (MHE) and solvent extraction (SE) on the yield, chemical composition, antibacterial and antibiofilm activity of essential oil were evaluated. The optimized conditions determined by response surface methodology were as follows: solvent to solid ratio was 5.2:1 (ml/g), extraction time was 15.5 min, and microwave...
