期刊论文

Comparative study on subcellular localization of swine-and canine-derived ELF4 protein

中文

畜牧与兽医

0529-5130

2024

56

3

45-53

cxcy2022001:国家级大学生创新创业训练计划项目 2021JJ30060:湖南省自然科学基金面上项目 21A0442:湖南省教育厅科学研究项目 2021NK2026:湖南省重点研发计划项目 2022ZYC091:中央引导地方科技发展资金项目

本校为第一机构

本研究旨在对比分析猪E74 样因子 4(sELF4)和犬E74 样因子 4(cELF4)的亚细胞定位,为深入开展二者功能研究提供依据.利用PCR截短扩增sELF4 和cELF4,分别克隆至真核表达载体pEGFP-C1 中,构建 17 个sELF4 重组真核表达载体和 13 个cELF4 重组真核表达质粒,转染HeLa细胞、Hoechst33342 染色,荧光倒置显微镜观察亚细胞定位,运用生物信息学软件分析其编码氨基酸序列特征.结果表明,sELF4 和cELF4 蛋白全长均定位在细胞核中,sELF4 196～291 aa(586～873 bp)之间有可能存在两个细胞质核定位信号肽,cELF4 的核定位信号肽分布在203～291 aa(607～873 bp),cELF4 292～663 aa(874～1 992 bp)、sELF4 292～662...

This study was to compare and analyze the subcellular localization of swine E74-like factor 4(sELF4)and canine E74-like fac-tor 4(cELF4),so as to provide a basis for further functional research on the two proteins.PCR was used here to truncate sELF4 and cELF4,which were cloned into the eukaryotic expression vector pEGFP-C1,separately.17 sELF4 recombinant eukaryotic expression vectors and 13 cELF4 recombinant eukaryotic expression plasmids were constructed,transfected into HeLa cells and stained with Hoechst33342.Subcellular localization was observed by fluorescence inverted microscope,and bioi...