期刊论文

中文

湖南农业科学

1006-060X

2015

9

5-7+11

10.16498/j.cnki.hnnykx.2015.09.002

湖南省自然科学基金省市联合基金（14JJ5001）；

本校为第一机构

为更好地利用啤酒糟,从富含纤维素的土壤中分离和筛选纤维素高效降解的曲霉,在啤酒糟培养基中加入不同的N源、C源,并调整其比例,接种等量的活化曲霉孢子悬浮液,观察菌丝生长情况以及测定菌株的酶活力,筛选促进菌株生长的最优配方。结果表明,最适的碳源和氮源分别为乳糖和胰蛋白胨,其最佳添加量分别为6%和4%;促进曲霉生长的最优碳氮比为5︰7,发酵后维素酶活力达188.49 IU,葡萄糖含量为0.38 g。这表明啤酒糟可以作为曲霉的优良培养基,对选育优良的啤酒糟纤维素分解菌具有一定的指导意义。

In order to make better use of brewer＇s grain, Aspergillus which degraded cellulose effectively was separated and screened from cellulose-rich soil; and the mycelial growth was observed and the enzyme activity of strains was determined to screen the optimal formula which promoted the growth of the strains by adding different C sources and N sources at different proportions of them to brewer＇s grain medium inoculated with same amount of activated Aspergillus spore suspension in this study. The results showed that the optimal C source was lacto...