Poly-L-lactic acid and silk protein powder as selective carbon, nitrogen for isolation of rare actinomycetes is worth further researches. Marine habitats are rich in rare actinomycetes with bioactivity and may be an effective method of ifnding new medicine. This study was aimed to isolate and identify rare actinomycetes with bioactivity isolated from marine habitats. Rare actinomycetes were isolated using poly-L-lactic acid and silk protein as a selective carbon, nitrogen from six samples by agar plate dilution method. To test the biological active effects, we used paper-disk method with seven...