期刊论文

Spectrophotometric Determination of Tea Saponin in Camellia Products

中文

山东化工

1008-021X

2022

51

17

101-104

10.3969/j.issn.1008-021X.2022.17.029

2021JJ50078:湖南省自然科学基金资助项目 2022KJH14:湖南省市场监督管理局资助项目 No.19K011:湖南省高校创新平台开放基金项目 :衡阳市科技创新计划项目

本校为第一机构

目的:建立一种适用油茶产品中提取和测定茶皂素的标准方法。方法:以油茶籽饼粕为原料，利用加热回流法提取茶皂素，通过优化实验条件，建立以香草醛-浓硫酸为显色剂的分光光度法测定茶皂素的检测方法。结果:茶皂素在为0.02～0.18 mg/m L质量浓度范围线性良好，其线性回归方程为A=4.853 6C-0.003 1（r=0.999 0），高、低、中不同水平加标回收率为81.5%～106.8%，方法检出限为0.002 5 mg/m L。结论:通过加标萃取率、加标回收率、精密度与检出限试验研究，建立了对一种茶皂素简便、快速、有效测定的标准方法。

Objective:To establish a standard method for extraction and determination of tea saponin from camellia products.Methods:tea saponin was extracted fromoil-tea Camellia seed cakeandmeal by heating reflux method.By optimizing the experimental conditions,a spectrophotometric method for the determination of tea saponin with vanillin-concentrated sulfuric acid as chromogenic agent was established.Results:The method had good linear relationship in 0.02~0.18 mg/mL and the linear regression equation was A=4.8536C-0.0031(r=0.9990),the recoveries of tea saponin at high,medium and low levels were 81.5%~10...