期刊论文

Behavioral and electrophysiological responses of Pieris rapae larvae cultured on Brassica oleracea L. to leaf saps of two host plants

中文

南方农业学报

2095-1191

2017

48

7

1225-1230

10.3969/j.issn.2095-1191.2017.07.16

31201750:国家自然科学基金 2016JJ4009:湖南省自然科学基金 16C0225:湖南省教育厅科学基金项目 15A03:衡阳师范学院科学基金

本校为第一机构

【目的】探讨小菜粉蝶幼虫对2种寄主植物(甘蓝和旱金莲)叶片汁液的行为及电生理反应,为阐明植食性昆虫寄主植物选择机制提供一定的理论依据。【方法】小菜粉蝶幼虫孵化后饲养在甘蓝叶片上,选用进入5龄24~48 h的小菜粉蝶幼虫供试;植物叶片榨出汁液,用行为试验双选法测试幼虫对汁液的行为反应,用顶端记录法测试幼虫侧栓锥和中栓锥味觉感受器对汁液的电生理反应。【结果】行为试验结果表明,甘蓝饲养小菜粉蝶幼虫取食含有甘蓝叶片汁液的玻璃纤维滤纸圆片极显著多于取食含有旱金莲叶片汁液的圆片(P

[Objective]Behavioral and electrophysiological responses of Pieris rapae larvae to two natural host plants (Brassica oleracea L. var. gemmifera cv. Cyrus L. and Tropaeolum majus L. cv. Glorious Gleam) saps in order to pro-vide reference for host selection mechanism of phytophagous insects. [Method]After P. rapae larvae were hatched, they were cultured on B. oleracea L. leaves, then the larvae growing into 5th instar for 24-28 h were used for the experiment. The leaves were squeezed to obtain the sap, and double-choice behavior experiment was used to test behavioral responses of the larvae to s...