期刊论文

中文

实用预防医学

1006-3110

2019

26

4

500-502

10.3969/j.issn.1006-3110.2019.04.034

湖南省自然科学基金会科卫联合项目（2018JJ6100）；

本校为其他机构

目的 了解湖南省美沙酮门诊就诊者感染丙型肝炎病毒(HCV)基因型特征. 方法收集湖南省内14个市州18岁以上、未接受抗病毒治疗、既往HCV抗体阳性的美沙酮门诊患者的血浆样本,每市州样本15份,共210份.对收集到的血浆样本分别进行HCV抗体EHSA复检、荧光PCR法定量HCV-RNA检测和基因分型检测. 结果在210名调查对象中,ELISA法复检HCV抗体阳性标本202例,阳性率96.19％;荧光PCR法检出HCV-RNA病毒载量＞15 IU/ml标本181例,阳性率86.19％.HCV基因型分别为1型18例(9.94％),2/3型51例(28.18％),1型混合2/3型47例(25.97％),其他型别65例(35.91％).区域分布以非1型为主,2/3型普遍分布. 结论 湖南省HCV基因型比较...

Objective To investigate the characteristics of HCV genotypes of outpatients in methadone maintenance treatment clinics in Hunan Province. Methods A total of 210 plasma samples were collected from outpatients aged above 18 years, without antiviral therapy and with positive anti-HCV antibody in methadone maintenance treatment clinics in 14 cities/prefectures in Hunan Province, and 15 plasma samples were collected from each city/prefecture. All the samples were tested with HCV antibody ELISA, HCV-RNA fluorescent quantitative polymerase chain reaction(FQ-PCR) method and genotyping assay. Results ...