FIASCO （Fast Isolation by AFLP Sequences Containing repeats） technique and magnetic bead enrichment method were used to develop microsatellite loci of the mud crab Scylla paramamosain. The results showed that more microsatellite sequences distributed within the range of 400bp in length and the probabilities of perfect microsatellite increased from di-to pentamer repeated types. 28 primer pairs were designed to amplify a sampling population, while 12 polymorphic loci were amplified, whose length conformed to theory size basically. Ten loci dis...
