This paper proposed the method of designing linked storage structure of the binary tree in DNA computer, which utilized the biological characteristics of DNA molecules and ligases. The linked storage structure of the binary tree was formally described. Being affected by ligase, among the various nodes generated hybridizations and linking reaction to form doublestranded DNA. All the biological technology mentioned could be practically implemented in the laboratory. To prove the feasibility of this method,gave out an instance of a binary tree' s...