In order to improve the output with high protein in Brewer‘s Grains with fermentation,take the Brewer‘s Grains as raw materials which through first fermentation with Aspergillus and Trichoderma,the crude protein content as the index, secondary fermentation with mixed Bacillus subtilis and Beer Yeast,and optimized the craftwork with response surface analysis.Results show that:the secondary fermentation with beer yeast and Bacillus subtilis in optimum conditions which tem-perature was 35 ℃,inoculum was 16.26 %,beer yeast and Bacillus subtilis ...