期刊论文

Evaluation of antioxidant activity of Lactobacillus in vivo using Caenorhabditis elegans and comparison with its antioxidant parameters in vitro

Wang, G.

中文

食品与发酵工业

0253-990X

2019

45

3

21-27

10.13995/j.cnki.11-1802/ts.018228

国家自然科学基金面上项目(31671839) 江苏高校品牌专业建设工程项目(PPZY2015A052) 国家自然科学基金青年项目(31601444) 湖南省教育厅资助科研项目(15B034).

本校为其他机构

通过对24株乳酸菌（Lactobacillus）羟自由基清除能力、DPPH自由基清除能力以及还原能力的测定,比较其体外抗氧化能力的大小,而后将乳酸菌喂食秀丽隐杆线虫（Caenorhabditis elegans）,并用质量分数为0. 003%的过氧化氢对线虫进行刺激,比较喂食不同乳酸菌的线虫寿命,分析乳酸菌在线虫体内缓解氧化损伤的能力。结果显示,植物乳杆菌（L. plantarum） Lp11不仅具有较高的体外抗氧化能力,还能有效缓解线虫体内因过氧化氢刺激引起的氧化应激,且缓解效果最好。体外抗氧化指标与线虫LT50值的相关性分析结果显示羟自由基清除能力这一体外抗氧化指标与缓解线虫氧化应激的效果具有较强相关性（P

The scavenging effects of twenty-four strains of Lactobacillus on hydroxyl radicals, DPPH free radicals, and their reducing activities were investigated to compare their antioxidative activities in vitro. Then, Caenorhabditis elegans fed with these twenty-four Lactobacillus strains were put into a 0.003%(mass froution) hydrogen peroxide solution that induced oxidative stress. By comparing the lifespan of C. elegans fed with different Lactobacillus strains, abilities of Lactobacilli to mitigate oxidative damages in C. elegans were analyzed. The ...