期刊论文

Lactobacillus plantarum with high cell adhesion and biofilm formation ability effectively inhibits the transcriptional activities of Campylobacter jejuni virulence genes in mice

中文

食品与发酵工业

0253-990X

2020

46

14

12-18

10.13995/j.cnki.11-1802/ts.023559

31671839:国家自然科学基金 31601444:国家自然科学基金 31301407:国家自然科学基金 15B034:湖南省教育厅科学研究项目 2019JJ50014:湖南省自然科学基金

本校为其他机构

该研究考察了12株植物乳杆菌(Lactobacillus plantarum)的生物学特性,包括其生长速率、产酸能力、对HT-29细胞的黏附能力、形成生物膜的能力、表面疏水性以及耐酸耐胆盐的能力,同时,将这12株植物乳杆菌分别干预被空肠弯曲杆菌(Campylobacter jejuni)和弓形虫复合感染的小鼠并考察其对宿主体内空肠弯曲杆菌毒力基因转录水平的影响。结果显示,植物乳杆菌N36和ZL4可以显著降低小鼠体内空肠弯曲杆菌毒力基因的转录水平。将植物乳杆菌生物学特性和其干预后小鼠体内空肠弯曲杆菌毒力基因转录变化进行相关性分析后发现,菌体表面高疏水性所带来的高细胞黏附性及较强的菌株生物膜形成能力使得其能在小鼠体...

In this study,the biological characteristics of 12 Lactobacillus plantarum strains were evaluated,including their growth rate,ability of acid production,adhesion to HT-29 cells,ability to form biofilms,surface hydrophobicity,and acid and bile salt resistance. Meanwhile,these strains were administered to Campylobacter jejuni and Toxoplasma gondii co-infected mice by gavage. The results showed that Lactobacillus plantarum N36 and ZL4 could significantly reduce the transcriptional activities of Campylobacter jejuni virulence genes. The correlation analysis of biological characteristics of Lactoba...