期刊论文

Lactobacillus salivarius CCFM 1054 alleviates the infection of Campylobacter jejuni in mice by regulating the gut microbiota

中文

食品与发酵工业

0253-990X

2020

46

5

1-8

10.13995/j.cnki.11-1802/ts.023280

31671839:国家自然科学基金 31601444:国家自然科学基金 31301407:国家自然科学基金 15B034:湖南省教育厅科学研究项目 2019JJ50014:湖南省自然科学基金

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考察了唾液乳杆菌(Lactobacillus salivarius)CCFM 1054体外培养产酸及发酵上清液抑制空肠弯曲杆菌(Campylobacter jejuni)生长能力、对人工模拟胃肠液中的耐受、对共培养条件下的抑菌能力、对HT-29细胞的粘附以及自我形成生物膜的能力,并以鼠李糖乳杆菌LGG和植物乳杆菌N49作为对比菌株,分别干预空肠弯曲杆菌和弓形虫复合感染的小鼠。结果显示,CCFM 1054能显著改变小鼠肠道菌群的组成,降低空肠弯曲杆菌在小鼠体内的定植率并缓解其感染。肠道菌群变化和乳酸菌拮抗空肠弯曲杆菌相关的体内外特性的相关性分析表明,CCFM 1054对细胞的高粘附性及其较强的生物膜形成能力使得其能在小鼠体内显著改变肠道...

In this study,the biological characteristics of Lactobacillus salivarius CCFM 1054 in vitro were evaluated,such as the ability of acid production,the antibacterial ability of free supernatant for Campylobacter jejuni growth,the tolerance to artificial gastrointestinal fluids,the inhibition to co-cultured C.jejuni,the adhesion to HT-29 cell and the ability of biofilms generation.It was founded that CCFM 1054 had strong acid-producing ability and could inhibit the growth of C.jejuni in vitro.At the same time,it had good tolerance under artificial simulated gastrointestinal fluid,high adhesion to...