期刊：
Entomologia Experimentalis et Applicata,2021年169(11):1039-1048 ISSN：0013-8703
通讯作者：
Jiu-Mei Long
作者机构：
[Teng, Tao; Liu, Jian-Hui; Zhou, Dong-Sheng; Long, Jiu-Mei] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang, Peoples R China.
通讯机构：
[Jiu-Mei Long] H;Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences and Environment, Hengyang Normal University, Hengyang, China
摘要：
<jats:title>Abstract</jats:title><jats:p>Habituation (accepting food sources previously rejected) and cross‐habituation (accepting food sources containing deterrents after exposure to unrelated deterrents) induced by dietary experience are observed in lepidopteran larvae, but the physiological mechanisms underlying these phenomena are poorly understood. In this study, behavioural and electrophysiological responsiveness to three unrelated deterrents were investigated in larvae of <jats:italic>Agrotis ipsilon</jats:italic> (Hufnagel) (Lepidoptera: Noctuidae). Caterpillars were fed with one of four diets: ‘normal’ artificial diet or diet with low amounts of the alkaloid strychnine, the phenolic chlorogenic acid (CA), or the diterpene fumaropimaric acid (FA). These three chemicals are structurally different and are all deterrent to <jats:italic>A. ipsilon</jats:italic> caterpillars. In behavioural, dual‐choice assays with chemical‐treated leaf discs, caterpillars reared on normal diet fed less on treated discs, i.e., were highly deterred by the three chemicals. However, caterpillars fed on chemical‐infused diets did not distinguish between treated and non‐treated discs, i.e., they showed behavioural habituation. In triple‐choice leaf disc assays, larvae fed with CA were deterred by both CA and FA and vice versa, i.e., larvae fed diet with CA or FA showed cross‐habituation. There was no cross‐habituation between strychnine and CA/FA. Electrophysiological recordings revealed that the same deterrent neuron in the lateral sensilla styloconica responds to CA and FA. Desensitisation of this neuron, through exposure to CA or FA in diet, was correlated with the observed cross‐habituation. A different deterrent neuron, in the medial sensilla styloconica, responded to strychnine. Thus, no generalisation of either habituation or desensitisation occurred to deterrents that elicited responses from different deterrent neurons in the lateral and the medial sensilla styloconica. Cross‐habituation to deterrents correlated with desensitisation of the corresponding deterrent neuron.</jats:p>
摘要：
Abstract Pimpinella smithii Wolff is endemic in China. In this study, the complete chloroplast genome of P. smithii was determined. The total genome size was 147,130 bp in length. It was found to possess the quadripartite structure, containing a pair of inverted repeats (IRs) of 18,264 bp, which were separated by large single copy (LSC) and small single copy (SSC) of 93,158 bp and 17,444 bp, respectively. A total of 129 genes were identified, including 85 protein-coding genes, 36 tRNA genes, and eight rRNA genes. The maximum-likelihood phylogenetic analysis indicated that P. smithii has close relationship with genus Angelica.
摘要：
The dual-choices tests of behavioral test were used to study the gustatory behavioral responses to caffeine of Helicoverpa armigera larvae and H. assulta larvae. Electrophysiological responses were studied by electrophysiological tip recording. Behavioral experiments showed that caffeine was a feeding deterrent for both larvae,but it showed a stronger feeding deterring effect on the oligophagous H. assulta. The electrophysiological tip-recording results showed that both H. armigera larvae and H. assulta larvae had one caffeine-sensitive feeding deterrent neuron at the medial sensilla,and the electrophysiological pulse response of H. assulta larvae was significantly stronger than that of H. armigera larvae. Therefore,caffeine had a stronger feeding deterring effect on the oligophagous H. assulta than the polyphagous H. armigera. The difference in behavioral effect was closely related to the sensitivity of to caffeine the feeding deterrent neurons at the medial sensilla.
