期刊：
Journal of Advanced Research,2023年54:133-146 ISSN：2090-1232
通讯作者：
Liu, JB;Fu, D;Ma, YS
作者机构：
[Diao, Xun; Liu, Ji-Bin; Liu, JB; Li, Sha; Fan, Qi-Yu] Nantong Univ, Inst Oncol, Affiliated Tumor Hosp, Nantong 226631, Jiangsu, Peoples R China.;[Li, Sha] Cent South Univ, Hunan Canc Hosp, Dept Head & Neck Surg, Cent Lab, Changsha 410013, Hunan, Peoples R China.;[Li, Sha] Cent South Univ, Xiangya Sch Med, Affiliated Canc Hosp, Changsha 410013, Hunan, Peoples R China.;[Xue, Pei] Shanghai Jiao Tong Univ, Sch Med, Dept Gen Surg, Div Gastrointestinal & Colorectal Surg,Ruijin Hosp, Shanghai 200025, Peoples R China.;[Ye, Kun] Cent South Univ, Xiangya Hosp 2, Dept Urol, Changsha 410011, Hunan, Peoples R China.
通讯机构：
[Liu, JB ] N;[Fu, D ; Ma, YS ] S;Nantong Univ, Inst Oncol, Affiliated Tumor Hosp, Nantong 226631, Jiangsu, Peoples R China.;Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Gen Surg,Inst Pancreat Dis, Shanghai 200025, Peoples R China.;Shanghai Univ Tradit Chinese Med, Longhua Hosp, Canc Inst, Shanghai 200032, Peoples R China.
关键词：
LIHC;Tumor;Treatment;Biomarker;Therapy
摘要：
Background: Aberrations in MYC underlie a large proportion of liver hepatocellular carcinoma (LIHC) cases; however, MYC is difficult to target because of its undruggable structure. We aimed to uncover MYC-associated molecular targets to provide new strategies for LIHC treatment. Methods: LIHC transcriptome datasets and clinical information were obtained from The Cancer Genome Atlas. A series of bioinformatics analyses were performed for 370 patients who were stratified based on the median MYC expression level (high-MYC group and low-MYC group). Correlation analysis was performed to determine relationships between the expression of key MYC-associated genes and prognosis, DNA promotor methylation, and immune cell infiltration. Gene ontology and Kyoto Encyclopedia of Genes and Genomes Pathway enrichment analyses were performed to elucidate the functions of these genes in LIHC. Their expression and functions in LIHC were further verified using transgenic mice overexpressing c-Myc under control of the hepatocyte-specific promoter (Alb-Cre). Results: AURKB, CCNB2, and CDKN3 were overexpressed in LIHC patients with high MYC expression and were associated with poor prognosis. Upregulation of these 3 genes was significantly correlated with hypomethylated promoter status, advanced T stage, metastasis, and immune cell infiltration in LIHC patients. Functional enrichment analyses indicated that these genes participate in the "p53 signaling pathway" and "cell cycle". Furthermore, RT-PCR and IHC analysis revealed that their mRNA and protein expression levels were upregulated in an Alb-Cre;cMYClsl/- mouse model. Drugs that target these 3 MYCrelated genes were identified. Conclusion: Taken together, our results identify biomarkers of potential utility for managing liver cancer therapy owing to their significance in tumorigenesis, proliferation, and tumor immunity. (c) 2023 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article
期刊：
Annals of Medicine,2022年54(1):2785-2795 ISSN：0785-3890
通讯作者：
Fu, D.;Cao, Y.-F.;Ma, Y.-S.
作者机构：
[Diao, Xun; Li, Sha; Yu, Xue-Chen; Fan, Qi-Yu; Liu, Ji-Bin] Nantong Univ, Affiliated Tumor Hosp, Inst Oncol, Nantong, Peoples R China.;[Yu, Xue-Chen] Macalester Coll, Dept Math Stat & Comp Sci, St Paul, MN USA.;[Tang, Qing-Hai] Hengyang Normal Univ, Hunan Key Lab Conservat & Utilizat Biol Resource, Hengyang, Peoples R China.;[Tang, Qing-Hai] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang, Peoples R China.;[Tang, Xiao-Mei; Fu, Da; Huang, Zhong-Yan] Shanghai Jiao Tong Univ, Ruijin Hosp, Gen Surg, Sch Med, Shanghai, Peoples R China.
通讯机构：
[Yong-Feng Cao] D;[Yu-Shui Ma] C;[Da Fu] G;Department of Oncology, Affiliated Tumor Hospital of Nantong University, Nantong, China<&wdkj&>Cancer Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China<&wdkj&>General Surgery, Ruijin Hospital & Institute of Pancreatic Diseases, Shanghai Jiaotong University School of Medicine, Shanghai, China<&wdkj&>Central Laboratory for Medical Research, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China
作者机构：
[Gu, Biao] Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China. Electronic address: biaogu@hynu.edu.cn;[Liu, Mengqin; Ye, Xinrong] Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China;[Long, Jiumei] College of Life Sciences and Environment, Hengyang Normal University, Hengyang 421008, PR China;[Xu, Zhifeng] Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China. Electronic address: xuzhifenghwl@163.com;[Shen, Youming] College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China. Electronic address: ymshen79@huas.edu.cn
通讯机构：
[Biao Gu; Zhifeng Xu] H;[Youming Shen] C;Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China<&wdkj&>College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China
摘要：
Nitric oxide (NO), an essential biological messenger molecule, participates in various physiological and pathological processes. The sensitive and specific detection of NO is of great significance for understanding the biological function of NO. Here, we synthesized a fluorescent probe (Rho-NO) for highly selective detection of NO both in vitro and in vivo. The high selectivity of Rho-NO is attributed to the fact that NO is easily replaced by electron donor amino group to form N-nitrosation products, causing rhodamine spiro ring open and fluorescence emit. Rho-NO showed a good linear response to NO (0-100 mu M) with a low detection limit (0.06 mu M). Importantly, it exhibited excellent specificity for NO detection in human serum and was also applied for imaging NO in living cells and inflammatory model of zebrafish. This work proves the potential of Rho-NO in pathological research and disease diagnosis. (C) 2021 Elsevier B.V. All rights reserved.
期刊：
Journal of Ethnopharmacology,2022年293:115329 ISSN：0378-8741
通讯作者：
Shihai Xing<&wdkj&>Weimin Jiang
作者机构：
[Guo, Xiaohu; Xing, Shihai; Yao, Xiaoyan; Cao, Mengyang; Wu, Jing; Peng, Daiyin; Peng, Huasheng; Meng, Fei] Anhui Univ Chinese Med, Coll Pharm, Hefei 230012, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, Minneapolis, MN 55108 USA.;[Jiang, Weimin; Li, Yan] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Peoples R China.;[Qu, Changqing] Fuyang Normal Univ, Engn Technol Res Ctr Antiaging, Chinese Herbal Med, Fuyang 236037, Peoples R China.;[Liu, Yingying] Anhui Univ Chinese Med, Coll Humanities & Int Educ Exchange, Hefei 230012, Peoples R China.
通讯机构：
[Shihai Xing] C;[Weimin Jiang] H;College of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China<&wdkj&>Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Hefei, 230038, China<&wdkj&>Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei, 230012, China<&wdkj&>Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences and Environment, Hengyang Normal University, Hengyang, 421008, China
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Salvia miltiorrhiza Bunge is a bulk medicinal material used in traditional Chinese medicine, that can cure cardiovascular diseases, neurasthenia, and other conditions. Sweating is a frequently used method of processing S. miltiorrhiza for medical applications. We previously demonstrated changes to the metabolic profile of linoleic acid, glyoxylate, and dicarboxylate after Sweating. However, this alteration has not been explained at the molecular level. MATERIALS AND METHODS: Fresh roots of Salvia miltiorrhiza Bunge were treated by the Sweating processing, and then the tandem mass tag technique was used to compare the proteome difference between Sweating S. miltiorrhiza and non-Sweating S. miltiorrhiza. RESULTS: We identified a total of 850 differentially expressed proteins after Sweating treatment in S. miltiorrhiza, including 529 upregulated proteins and 321 downregulated proteins. GO enrichment analysis indicated that these differentially expressed proteins are involved in external encapsulating structure, cell wall, oxidoreductase activity, ligase activity, and others. Further analysis showed that CYP450, the pathogenesis-related protein Bet v 1 family, and the peroxidase domain were the major protein domains. KEGG enrichment identified 18 pathways, of which phenylpropanoid biosynthesis is the most important one related to the metabolite profile and is the principal chemical component of S. miltiorrhiza. CONCLUSION: This study addressed potential molecular mechanisms in S. miltiorrhiza after Sweating, and the findings provide reasons for the changes in biochemical properties and metabolites changes which might cause pharmacological variation at the proteome level.
摘要：
Toxoplasmosis is a widely distributed parasitic protozoan disease, caused by Toxoplasma gondii (T. gondii). High prevalence of toxoplasmosis and limitations of conventional treatments lead to a search for new therapeutic drugs. Lycosin-I is a linear peptide, derived from the venom of the spider Lycosa singoriensis. The aim of the present study was to determine the anti-parasitic effect of lycosin-Iota against T. gondii. In vitro, the anti-T. gondii activities of lycosin-Iota were evaluated by MTT assay, trypan blue exclusion assay, cell counting assay and plaque assay. Cytokines of IL-6 and IL-8 were measured by quantitative PCR. In addition, the structures of tachyzoites treated with lycosin-Iota were also observed by scanning and transmission electron microscopy. In vivo, mice were challenged with parasites treated by lycosin-I. The results revealed that lycosin-Iota had shown a significant ability to inhibit T. gondii invasion and proliferation. Cytokines of IL-6 and IL-8 were reduced by lycosin-Iota at transcription level in human foreskin fibroblast (HFF) cells infected with T. gondii tachyzoites, but they were increased compared to non-infected cells. For tachyzoites, lycosin-Iota induced their cell membrane alterations with formation of invaginations, some of them appeared to be vacuolated in their cytoplasm. Moreover, lycosin-Iota had prolonged the survival time of mice by controlling T. gondii proliferation. In conclusion, our present study provides the first evidence for anti-T. gondii by using the spider peptide lycosin-Iota. These findings suggest that lycosin-Iota is a potential alternative agent for the treatment of toxoplasmosis.
摘要：
Although isomer-specific bioaccumulation of dechlorane plus (DP) has been addressed in many studies, it remains unclear which factors determine this process and whether biotransformation of DP occurs in organisms. Comparative experiments were conducted in both in vivo and in ovo incubation using hens and eggs to identify the dominant factors determining the bioaccumulation of DP. Hens and fertilized eggs were exposed to DP isomers (syn- and anti-DP) by feeding and spiking, respectively, to investigate absorption, elimination, and metabolism. No significant differences were found between absorption efficiencies of DP isomers in the adult hens. Following first-order kinetics, anti-DP exhibited a slightly longer half-life than syn-DP as well as an elevated anti-DP fraction in laid eggs, thereby suggesting selective enrichment of anti-DP in adult hens. However, chicken embryos metabolized approximately 12% and 28% of the absorbed syn- and anti-DP, respectively, thereby verifying that and-DP was preferably metabolized. This result indicated that stereo-selective excretion of syn-DP, rather than preferred metabolism of anti-DP, played a more prominent role in isomer-specific bioaccumulation of DP in chickens. Further studies on metabolites of DP are crucial to understanding the fate of DP in organisms. (C) 2019 Elsevier Ltd. All rights reserved.
作者机构：
[Mai, Bi-Xian; Luo, Yuan-Lai; Luo, Xiao-Jun; Zeng, Yan-Hong; Lin, Lan; Ye, Mei-Xia] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China.;[Mai, Bi-Xian; Luo, Yuan-Lai; Luo, Xiao-Jun; Zeng, Yan-Hong; Lin, Lan; Ye, Mei-Xia] Chinese Acad Sci, Guangzhou Inst Geochem, Guangdong Key Lab Environm Resources Utilizat & P, Guangzhou 510640, Guangdong, Peoples R China.;[Luo, Yuan-Lai] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Peoples R China.;[Lin, Lan; Ye, Mei-Xia] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
通讯机构：
[Luo, Xiao-Jun] C;Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China.;Chinese Acad Sci, Guangzhou Inst Geochem, Guangdong Key Lab Environm Resources Utilizat & P, Guangzhou 510640, Guangdong, Peoples R China.
关键词：
Deiodinase activity;Polybromodiphenyl ethers;Species-specific debromination;Teleost fish
摘要：
A combination of previous studies and the present study indicated species-specific debromination of polybromodiphenyl ethers (PBDEs) in teleost fish. Three situations of debromination were found, namely rapid debromination represented by debromination of BDE 99 to BDE 47 observed in common carp, tilapia, crucian carp, and oscar fish; slow debromination represented by debromination of BDE 99 to BDE 49 observed in the abovementioned fish and rainbow trout, salmon, and snakehead; and no or minor debromination observed in catfish. The results of experiments on cofactors, inhibitors, and substrate competitors indicated that activities of outer ring deiodinase of 3, 3', 5'-triiodothyronine (type I deiodinase), which cannot be inhibited by 6-propyl-2-thiouracil, were responsible for the rapid debromination, and the outer ring deiodinase of thyroxine (type II deiodinase) regulated the slow debromination. The debromination of BDE 99 to BDE 49 was more common, but occurred at a much lower rate (approximately 100 times lower) than the debromination of BDE 99 to BDE 47. This was because the activity of type II deiodinase was nearly two orders of magnitude lower than that of type I deiodinase in the fish species studied. Further studies on debromination of PBDEs and properties of deiodinase in more species are needed to confirm the hypothesis. (C) 2019 Elsevier Ltd. All rights reserved.
摘要：
Aims & ScopeThe Journal of Consumer Policy is a refereed, international journal which encompasses a broad range of issues concerned with consumer affairs. It looks at the consumer's dependence on existing social and economic structures, helps to define the consumer's interest, and discusses the ways in which consumer welfare can be fostered &ndash; or restrained &ndash; through actions and policies of consumers, industry, organizations, government, educational institutions, and the mass media. The Journal of Consumer Policy publishes theoretical and empirical research on consumer and producer conduct, emphasizing the implications for consumers and increasing communication between the parties in the marketplace. Articles cover consumer issues in law, economics, and behavioural sciences. Current areas of topical interest include the impact of new information technologies, the economics of information, the consequences of regulation or deregulation of markets, problems related to an increasing internationalization of trade and marketing practices, consumers in less affluent societies, the efficacy of economic cooperation, consumers and the environment, problems with products and services provided by the public sector, the setting of priorities by consumer organizations and agencies, gender issues, product safety and product liability, and the interaction between consumption and associated forms of behaviour such as work and leisure. The Journal of Consumer Policy reports regularly on developments in legal policy with a bearing on consumer issues. It covers the integration of consumer law in the European Union and other transnational communities and analyzes trends in the application and implementation of consumer legislation through administrative agencies, courts, trade associations, and consumer organizations. It also considers the impact of consumer legislation on the supply side and discusses comparative legal approaches to issues of consumer policy in different parts of the world. The Journal of Consumer Policy informs readers about a broad array of consumer policy issues by publishing regularly both extended book reviews and brief, non-evaluative book notes on new publications in the field.Coverage in the Journals&commat;Ovid database begins with the Volume 20, Issue 1, 1997 issue.
