通讯机构：
[Wei Liu; Xuexiao Zou] C;[Zhuqing Zhang] V;College of Life Sciences and Environment, Hengyang Normal University, Hengyang 421008, China<&wdkj&>College of Horticulture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Longping Branch, Graduate School of Hunan University, Changsha 410125, China<&wdkj&>Vegetable Institution of Hunan Academy of Agricultural Science, Changsha 410125, China
摘要：
The stay-green trait is of considerable importance in extending the shelf life of green pepper fruit (Capsicum annuum L.) and in enhancing the appearance of ornamental plants. The study revealed the genetic and regulatory mechanisms of the stay-green trait in pepper, which will aid in the selection of ornamental pepper varieties. In this study, a pepper mutant with stay-green fruit named TNX348 was identified from a germplasm resource bank. Two segregating populations were constructed using the stay-green mutant TNX348 and then used in bulked segre-gant analysis combined with RNA sequencing and linkage analyses. The causal gene of the stay-green trait was mapped to an approximately 131-kb region, and a senescence-induced chloroplast protein gene, CaSGR1 (Capana01g000359), was identified as a candidate gene. Sequencing analysis revealed a G -> A single-base mutation of CaSGR1 in TNX348 that led to early termination of translation. Based on the single-base mu-tation, a single nucleotide polymorphism (SNP) marker co-segregating with the stay-green trait was developed. Furthermore, in transcriptome analysis, expression patterns of 11 hormone transduction-related transcription factors, such as abscisic acid-insensitive (ABI), abscisic acid-responsive element-binding factor (ABF), and NAC transcription factor, were similar or opposite to that of CaSGR1. The results indicated that the transcription factors might mediate chlorophyll degradation by regulating the expression of CaSGR1.
摘要：
Pimpinella L. is one of the large genera in the Apiaceae family. In a previous study, the molecular phylogenies of Pimpinella were explored using nuclear ribosomal DNA internal transcribed spacers (ITS) and several chloroplast DNA segments. There have been few studies conducted on chloroplast genomes in Pimpinella, which has limited systematic understanding of this genus. We assembled the complete chloroplast genomes of nine Pimpinella species from China using data generated from next generation sequencing (NGS). The chloroplast (cp) DNA used were standard double -stranded molecules, ranging from 146,432 base pairs (bp) (P. valleculosa) to 165,666 bp (P. purpurea) in length. The circular DNA contained a large single-copy (LSC) region, small single-copy (SSC) region, and pair of inverted repeats (IRs). The cp DNA of the nine species contained 82-93 protein-coding genes, 36-37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes, respectively. Four species (P. smithii, P. valleculosa, P. rhomboidea, and P. purpurea) exhibited striking distinctions in genome size, gene number, IR boundary, and sequence identity. We confirmed the non-monophyly of the Pimpinella species on the basis of the nine newly identified plastomes. The distant relationship between the above-mentioned four Pimpinella species and Pimpinelleae was indicated with high support values. Our study provides a foundation for future in-depth phylogenetic and taxonomic studies of genus Pimpinella.
摘要：
Arsenic (As) and cadmium (Cd) often coexist in paddy soils. Nano-ferrihydrite colloidal particles (NFPs) are ubiquitous at redox active interfaces of the paddy system and are well-known to play a critical role in controlling the solubility and bio-availability of As and Cd. However, the mutual interaction between As and Cd on NFPs remains elusive. Herein, batch experiments and in-situ spectroscopic techniques were used to investigate the effects of the interaction pattern (sequential reaction) of Cd(II) and As(V) on their respective adsorption on the surfaces of NFPs. Two scenarios were designed: Cd(II) pre-saturated NFPs and As(V) pre-saturated NFPs. Adsorption of Cd(II) was increased by 1.67, 4.08, and 5.21 times in As(V)-saturated NFPs, but only by 1.05, 1.11, and 1.15 times for As(V) in Cd(II)-saturated NFPs. Further, we determined the pH-dependent mutually beneficial cooperation pathways as mediated by the surface of NFPs. At lower pH (5), As(V) tended to promote Cd(II) adsorption, whereas Cd(II) tended to enhance As(V) adsorption at higher pH (> 5.5). X-ray photoelectron spectroscopy (XPS) indicated that both pre-saturated Cd(II) and As(V) altered the local coordination environment of their counterpart ions. Furthermore, results from in-situ attenuated total reflection-Fourier-transform infrared spectroscopy (ATR-FTIR) and second derivative peak shape fitting revealed two types of ternary surface complexes, namely Cd(II)-bridged and As(V)-bridged complexes, which were responsible for the distinct Cd(II) and As(V) co-adsorption behavior on the surface of NFPs under different conditions. These findings help us understand how co-presence Cd and As behave in an iron-rich geological setting and will aid in the development of related restoration technologies.
摘要：
Pseudomonas aeruginosa is a conditional Gram-negative pathogen that produces extracellular virulence factors that can lead to bloodstream invasion, severely harm tissues, and disseminate bacteria, ultimately leading to various diseases. In this study, lactic acid bacteria (LAB) with strong antagonistic ability against P. aeruginosa were screened, and the regulatory mechanism of LAB against P. aeruginosa was evaluated. The results showed that the three selected LAB strains had strong inhibition ability on the growth, biofilm formation, and pyocyanin expression of P. aeruginosa and a promoting effect on the expression of autoinducer-2. Among them, Lactipantibacillus plantarum (Lp. plantarum) LPyang is capable of affecting the metabolic processes of P. aeruginosa by influencing metabolic substances, such as LysoPC, oxidized glutathione, betaine, etc. These results indicate that LPyang reduces the infectivity of P. aeruginosa through inhibition of its growth, biofilm formation, pyocyanin expression, and regulation of its metabolome. This study provides new insights into the antagonistic activity of Lp. plantarum LPyang against P. aeruginosa.
摘要：
Simple Summary Habituation to deterrent plant compounds has been found in generalist and specialist insect herbivores. The rate at which plant-feeding insects habituate and at which sensitivity of taste neurons detecting deterrents changes has not been compared among closely related species. The generalist Helicoverpa armigera (Hubner) and the specialist Helicoverpa assulta (Guenee) (Lepidoptera: Noctuidae) differ strongly in degree of host-plant specialism. Both species habituated to the alkaloid strychnine after dietary exposure; however, the specialist H. assulta displayed habituation to strychnine faster (at 48 h) than the generalist H. armigera (at 72 h). Electrophysiological recordings from taste sensilla on mouthparts revealed that a deterrent-sensitive neuron in the medial sensillum styloconicum of both species displayed significantly reduced sensitivity to the deterrent that coincided in time with the onset of habituation. Our findings show that physiological changes in taste neuron sensitivity coincide with habituation to plant compounds that are initially avoided. The two closely related moth species, Helicoverpa armigera and H. assulta differ strongly in their degree of host-plant specialism. In dual-choice leaf disk assays, caterpillars of the two species that had been reared on standard artificial diet were strongly deterred by the plant-derived alkaloid strychnine. However, caterpillars of both species reared on artificial diet containing strychnine from neonate to the 5th instar were insensitive to this compound. Fifth instar caterpillars of H. assulta and 4th or 5th instars of H. armigera not exposed to strychnine before were subjected to strychnine-containing diet for 24 h, 36 h, 48 h, or 72 h. Whereas H. assulta displayed habituation to strychnine after 48 h, it took until 72 h for H. armigera to become habituated. Electrophysiological tests revealed that a deterrent-sensitive neuron in the medial sensillum styloconicum of both species displayed significantly reduced sensitivity to strychnine that correlated with the onset of habituation. We conclude that the specialist H. assulta habituated faster to strychnine than the generalist H. armigera and hypothesis that desensitization of deterrent-sensitive neurons contributed to habituation.
通讯机构：
Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Science, Changsha, Hunan, China
摘要：
To study the effects of dietary energy level on the meat quality of different muscles in finishing pigs, 400 Xiangcun Black pigs (BW 1/4 79.55 & PLUSMN; 4.77 kg) were randomly assigned to 5 treatments with varied calculated digestive energy (DE) at 3,050, 3,100, 3,150, 3,200 and 3,250 kcal/kg, respectively. Each treatment had 8 replicates with 10 pigs per replicate. Meat quality, amino acid and fatty acid composition were tested in this study. No differences in average daily gain, average daily feed intake or feed-to-gain ratio (P > 0.05) were observed among dietary treatments. Glycogen concentrations of longissimus dorsi (LD) muscle in DE3150 was higher than those in other groups (P < 0.05). The crude fat concentration of biceps femoris (BF) muscle in DE3250 tended to be higher than that in DE3150 and DE3100 groups (P < 0.05). Pigs in DE3250 and DE3200 had higher fiber density and smaller cross-sectional area of BF muscle than those in DE3150 (P < 0.05). Pigs in DE3150 had the highest Cu concentration in LD muscle compared with those in DE3200, DE3250 (P < 0.05). The C16:1 proportion of LD muscle was lower (P < 0.01) and C20:1 was higher (P < 0.05) in DE3050 than that in the other dietary treatments. The C18:3n6 and C20:3n6 proportions of BF muscle in DE3150 were higher than those in DE 3050, DE3200 and DE3250 (P < 0.05). For LD muscle, mRNA ex-pressions of type I and IIa MyHC in group DE3150 were higher than other treatments (P < 0.01). The LD muscle in DE3150 expressed higher PPARd than in other groups (P < 0.01). Pigs in DE3100 expressed higher FOX1 than in DE3200 and DE3250 (P < 0.05). Sterol-regulatory element binding proteins (SREBPa) mRNA expression decreased linearly when dietary energy level increased in BF muscle (P < 0.01). In conclusion, a 200 kcal/kg decrease in digestible energy for 4 consecutive weeks did not affect growth performance of Xiangcun Black pigs. Furthermore, LD and BF muscle respond differently to dietary energy level, and meat quality was improved by the medium energy level during the finishing phase. (c) 2022 Chinese Association of Animal Science and Veterinary Medicine. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
期刊：
Pakistan Journal of Botany,2022年54(5):1859-1864 ISSN：0556-3321
通讯作者：
Wang, Z.
作者机构：
[Wang, Zhixin; Cao, Limin; Liu, Jianhui] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Peoples R China.
通讯机构：
Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences and Environment, Hengyang Normal University, Hengyang, China
期刊：
Frontiers in Plant Science,2022年13:937392 ISSN：1664-462X
通讯作者：
Peng, D.
作者机构：
[Wu, Liping; Peng, Daiyin; Xing, Shihai; Su, Xinglong; Meng, Fei; Wang, Zhaojian] Anhui Univ Chinese Med, Coll Pharm, Hefei, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, St Paul, MN USA.;[Huang, Huizhen; Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang, Peoples R China.;[Liu, Yingying] Anhui Univ Chinese Med, Coll Humanities & Int Educ Exchange, Hefei, Peoples R China.;[Wang, Longhai] Anhui Univ Chinese Med, Sch Integrated Chinese & Western Med, Hefei, Peoples R China.
通讯机构：
College of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
关键词：
Proteome;phosphoproteome;stone planting under the forest;Greenhouse planting;Dendrobium huoshanense
摘要：
The highly esteemed Chinese herb, Dendrobium huoshanense, whose major metabolites are polysaccharides and alkaloids, is on the verge of extinction. The stone planting under the forest (SPUF) and greenhouse planting (GP) of D. huoshanense are two different cultivation methods of pharmaceutical Dendrobium with significantly differences in morphology, metabolites content and composition, and medication efficacy. Here, we conducted proteomics and phosphoproteomics analyses to reveal differences in molecular mechanisms between SPUF and GP. We identified 237 differentially expressed proteins (DEPs) between the two proteomes, and 291 modification sites belonging to 215 phosphoproteins with a phosphorylation level significantly changed (PLSC) were observed. GO, KEGG pathway, protein domain, and cluster analyses revealed that these DEPs were mainly localized in the chloroplast; involved in processes such as posttranslational modification, carbohydrate transport and metabolism, and secondary metabolite biosynthesis; and enriched in pathways mainly including linoleic acid metabolism, plant-pathogen interactions, and phenylpropanoid, cutin, suberin, and wax biosynthesis. PLSC phosphoproteins were mainly located in the chloroplast, and highly enriched in responses to different stresses and signal transduction mechanisms through protein kinase and phosphotransferase activities. Significant differences between SPUF and GP were observed by mapping the DEPs and phosphorylated proteins to photosynthesis and polysaccharide and alkaloid biosynthesis pathways. Phosphorylation characteristics and kinase categories in D. huoshanense were also clarified in this study. We analyzed different molecular mechanisms between SPUF and GP at proteomic and phosphoproteomic levels, providing valuable information for the development and utilization of D. huoshanense.
作者机构：
[Wu, Liping; Wang, Shuting; Guo, Xiaohu; Xing, Shihai; Su, Xinglong; Yao, Xiaoyan; Wang, Zhaojian; Wu, Jing; Zhang, Jing; Meng, Fei] Anhui Univ Chinese Med, Coll Pharm, Hefei 230012, Peoples R China.;[Xing, Shihai; Yao, Xiaoyan] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;[Sun, Zongping; Qu, Changqing] Fuyang Normal Univ, Anhui Prov Key Lab Environm Hormone & Reprod, Anhui Prov Key Lab Embryo Dev & Reprod Regulat, Fuyang 236037, Peoples R China.;[Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Hunan, Peoples R China.;[Wu, Jing] MOE Anhui Joint Collaborat Innovat Ctr Qual Impro, Hefei 230038, Peoples R China.
通讯机构：
[Shihai Xing] C;College of Pharmacy, Anhui Univ. of Chinese Medicine, Hefei, 230012 China<&wdkj&>Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, 230012 China<&wdkj&>Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei, 230012 China
摘要：
Ginger (Zingiber officinale Roscoe) is an important plant used worldwide for medicine and food. The R2R3-MYB transcription factor (TF) family has essential roles in plant growth, development, and stresses resistance, and the number of genes in the family varies greatly among different types of plants. However, genome-wide discovery of ZoMYBs and gene responses to stresses have not been reported in ginger. Therefore, genome-wide analysis of R2R3-MYB genes in ginger was conducted in this study. Protein phylogenetic relations and conserved motifs and chromosome localization and duplication, structure, and cis-regulatory elements were analyzed. In addition, the expression patterns of selected genes were analyzed under two different stresses. A total of 299 candidate ZoMYB genes were discovered in ginger. Based on groupings of R2R3-MYB genes in the model plant Arabidopsis thaliana (L.) Heynh., ZoMYBs were divided into eight groups. Genes were distributed across 22 chromosomes at uneven densities. In gene duplication analysis, 120 segmental duplications were identified in the ginger genome. Gene expression patterns of 10 ZoMYBs in leaves of ginger under abscisic acid (ABA) and low-temperature stress treatments were different. The results will help to determine the exact roles of ZoMYBs in anti-stress responses in ginger.
期刊：
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy,2022年278:121290 ISSN：1386-1425
通讯作者：
Gu, B.
作者机构：
[Gu, Biao] Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China. Electronic address: biaogu@hynu.edu.cn;[Liu, Mengqin; Ye, Xinrong] Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China;[Long, Jiumei] College of Life Sciences and Environment, Hengyang Normal University, Hengyang 421008, PR China;[Xu, Zhifeng] Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China. Electronic address: xuzhifenghwl@163.com;[Shen, Youming] College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China. Electronic address: ymshen79@huas.edu.cn
通讯机构：
Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, China
期刊：
Frontiers in Plant Science,2022年13:822374 ISSN：1664-462X
通讯作者：
Peng, Daiyin;Xing, SH;Peng, DY
作者机构：
[Xing, SH; Yao, Liang; Ye, Mengjuan; Xing, Shihai; Yao, Xiaoyan; Wang, Zhaojian; Wu, Jing; Zhang, Jing; Yu, Nianjun; Peng, Daiyin; Meng, Fei; Peng, DY] Anhui Univ Chinese Med, Coll Pharm, Hefei, Peoples R China.;[Xing, SH; Xing, Shihai; Wu, Jing; Yu, Nianjun; Peng, Daiyin; Peng, DY] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA.;[Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang, Peoples R China.;[Wang, Longhai] Anhui Univ Chinese Med, Sch Integrated Chinese & w Med, Hefei, Peoples R China.
通讯机构：
[Xing, SH ; Peng, DY] A;[Peng, DY ] S;Anhui Univ Chinese Med, Coll Pharm, Hefei, Peoples R China.;Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei, Peoples R China.;Synerget Innovat Ctr Anhui Authent Chinese Med Qu, Hefei, Peoples R China.
期刊：
Annals of Medicine,2022年54(1):2785-2795 ISSN：0785-3890
通讯作者：
Yong-Feng Cao<&wdkj&>Yu-Shui Ma<&wdkj&>Da Fu
作者机构：
[Diao, Xun; Li, Sha; Yu, Xue-Chen; Fan, Qi-Yu; Liu, Ji-Bin] Nantong Univ, Affiliated Tumor Hosp, Inst Oncol, Nantong, Peoples R China.;[Yu, Xue-Chen] Macalester Coll, Dept Math Stat & Comp Sci, St Paul, MN USA.;[Tang, Qing-Hai] Hengyang Normal Univ, Hunan Key Lab Conservat & Utilizat Biol Resource, Hengyang, Peoples R China.;[Tang, Qing-Hai] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang, Peoples R China.;[Tang, Xiao-Mei; Fu, Da; Huang, Zhong-Yan] Shanghai Jiao Tong Univ, Ruijin Hosp, Gen Surg, Sch Med, Shanghai, Peoples R China.
通讯机构：
[Yong-Feng Cao] D;[Yu-Shui Ma] C;[Da Fu] G;Department of Oncology, Affiliated Tumor Hospital of Nantong University, Nantong, China<&wdkj&>Cancer Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China<&wdkj&>General Surgery, Ruijin Hospital & Institute of Pancreatic Diseases, Shanghai Jiaotong University School of Medicine, Shanghai, China<&wdkj&>Central Laboratory for Medical Research, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China
关键词：
HCC;stage;SEER;treatment;prognosis
摘要：
BACKGROUND: Improvements in screening and imaging technologies and treatment of liver disease have influenced the trend in diagnosis for stage I liver cancer. In this article, recent trends in age, incidence, tumour size, and survival of different stages of liver cancer are analysed. METHODS: Surveillance, Epidemiology, and end results data from the National Cancer Institute were used to analyse trends in age-adjusted incidence rate, mean tumour size at diagnosis, age at diagnosis, and 5-year survival probability for stage I liver cancer. RESULTS: Stage I cases of liver cancer increased most tremendously over the study period, with a greater increase from 2004 to 2012 following a smaller increase from 2012 to 2015. Moreover, the mean age of stage I liver cancer increased by 1.72 years from 2004 to 2015. The 5-year-overall survival for stage I liver cases worsened from 97.9% to 83.7% from 2004 to 2011, whereas the 10-year survival probability for stage I cases worsened from 97.3% in 2004 to 79.6% in 2006. Comparing with higher stage cases, stage I liver cancer were more likely to be females, be married, live in metro areas, receive chemotherapy, and carry medical insurance. CONCLUSIONS: The incidence of stage I liver cancer has increased over the study period, with an increase in age of diagnosis, decrease in tumour size, and generally stable overall survival rate with slight decrease. These trends emphasized the importance of early detection of liver cancer and regular screening and better treatment for high-risk populations.RESEARCH HIGHLIGHTSImprovements in screening and imaging technologies and treatment of liver disease have influenced the trend in diagnosis for liver cancer.Stage I cases of liver cancer increased most tremendously over the study period, with a greater increase from 2004 to 2012 following a smaller increase from 2012 to 2015.These trends emphasized the importance of early detection of liver cancer and regular screening and better treatment for high-risk populations.
摘要：
Nitric oxide (NO), an essential biological messenger molecule, participates in various physiological and pathological processes. The sensitive and specific detection of NO is of great significance for understanding the biological function of NO. Here, we synthesized a fluorescent probe (Rho-NO) for highly selective detection of NO both in vitro and in vivo. The high selectivity of Rho-NO is attributed to the fact that NO is easily replaced by electron donor amino group to form N-nitrosation products, causing rhodamine spiro ring open and fluorescence emit. Rho-NO showed a good linear response to NO (0-100 mu M) with a low detection limit (0.06 mu M). Importantly, it exhibited excellent specificity for NO detection in human serum and was also applied for imaging NO in living cells and inflammatory model of zebrafish. This work proves the potential of Rho-NO in pathological research and disease diagnosis. (C) 2021 Elsevier B.V. All rights reserved.
