通讯机构:
[Wei Liu; Xuexiao Zou] C;[Zhuqing Zhang] V;College of Life Sciences and Environment, Hengyang Normal University, Hengyang 421008, China<&wdkj&>College of Horticulture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Longping Branch, Graduate School of Hunan University, Changsha 410125, China<&wdkj&>Vegetable Institution of Hunan Academy of Agricultural Science, Changsha 410125, China
摘要:
The stay-green trait is of considerable importance in extending the shelf life of green pepper fruit (Capsicum annuum L.) and in enhancing the appearance of ornamental plants. The study revealed the genetic and regulatory mechanisms of the stay-green trait in pepper, which will aid in the selection of ornamental pepper varieties. In this study, a pepper mutant with stay-green fruit named TNX348 was identified from a germplasm resource bank. Two segregating populations were constructed using the stay-green mutant TNX348 and then used in bulked segre-gant analysis combined with RNA sequencing and linkage analyses. The causal gene of the stay-green trait was mapped to an approximately 131-kb region, and a senescence-induced chloroplast protein gene, CaSGR1 (Capana01g000359), was identified as a candidate gene. Sequencing analysis revealed a G -> A single-base mutation of CaSGR1 in TNX348 that led to early termination of translation. Based on the single-base mu-tation, a single nucleotide polymorphism (SNP) marker co-segregating with the stay-green trait was developed. Furthermore, in transcriptome analysis, expression patterns of 11 hormone transduction-related transcription factors, such as abscisic acid-insensitive (ABI), abscisic acid-responsive element-binding factor (ABF), and NAC transcription factor, were similar or opposite to that of CaSGR1. The results indicated that the transcription factors might mediate chlorophyll degradation by regulating the expression of CaSGR1.
期刊:
Journal of Advanced Research,2023年54:133-146 ISSN:2090-1232
通讯作者:
Liu, JB;Fu, D;Ma, YS
作者机构:
[Diao, Xun; Liu, Ji-Bin; Liu, JB; Li, Sha; Fan, Qi-Yu] Nantong Univ, Inst Oncol, Affiliated Tumor Hosp, Nantong 226631, Jiangsu, Peoples R China.;[Li, Sha] Cent South Univ, Hunan Canc Hosp, Dept Head & Neck Surg, Cent Lab, Changsha 410013, Hunan, Peoples R China.;[Li, Sha] Cent South Univ, Xiangya Sch Med, Affiliated Canc Hosp, Changsha 410013, Hunan, Peoples R China.;[Xue, Pei] Shanghai Jiao Tong Univ, Sch Med, Dept Gen Surg, Div Gastrointestinal & Colorectal Surg,Ruijin Hosp, Shanghai 200025, Peoples R China.;[Ye, Kun] Cent South Univ, Xiangya Hosp 2, Dept Urol, Changsha 410011, Hunan, Peoples R China.
通讯机构:
[Liu, JB ] N;[Fu, D ; Ma, YS ] S;Nantong Univ, Inst Oncol, Affiliated Tumor Hosp, Nantong 226631, Jiangsu, Peoples R China.;Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Gen Surg,Inst Pancreat Dis, Shanghai 200025, Peoples R China.;Shanghai Univ Tradit Chinese Med, Longhua Hosp, Canc Inst, Shanghai 200032, Peoples R China.
关键词:
LIHC;Tumor;Treatment;Biomarker;Therapy
摘要:
Background: Aberrations in MYC underlie a large proportion of liver hepatocellular carcinoma (LIHC) cases; however, MYC is difficult to target because of its undruggable structure. We aimed to uncover MYC-associated molecular targets to provide new strategies for LIHC treatment. Methods: LIHC transcriptome datasets and clinical information were obtained from The Cancer Genome Atlas. A series of bioinformatics analyses were performed for 370 patients who were stratified based on the median MYC expression level (high-MYC group and low-MYC group). Correlation analysis was performed to determine relationships between the expression of key MYC-associated genes and prognosis, DNA promotor methylation, and immune cell infiltration. Gene ontology and Kyoto Encyclopedia of Genes and Genomes Pathway enrichment analyses were performed to elucidate the functions of these genes in LIHC. Their expression and functions in LIHC were further verified using transgenic mice overexpressing c-Myc under control of the hepatocyte-specific promoter (Alb-Cre). Results: AURKB, CCNB2, and CDKN3 were overexpressed in LIHC patients with high MYC expression and were associated with poor prognosis. Upregulation of these 3 genes was significantly correlated with hypomethylated promoter status, advanced T stage, metastasis, and immune cell infiltration in LIHC patients. Functional enrichment analyses indicated that these genes participate in the "p53 signaling pathway" and "cell cycle". Furthermore, RT-PCR and IHC analysis revealed that their mRNA and protein expression levels were upregulated in an Alb-Cre;cMYClsl/- mouse model. Drugs that target these 3 MYCrelated genes were identified. Conclusion: Taken together, our results identify biomarkers of potential utility for managing liver cancer therapy owing to their significance in tumorigenesis, proliferation, and tumor immunity. (c) 2023 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article
摘要:
Pimpinella L. is one of the large genera in the Apiaceae family. In a previous study, the molecular phylogenies of Pimpinella were explored using nuclear ribosomal DNA internal transcribed spacers (ITS) and several chloroplast DNA segments. There have been few studies conducted on chloroplast genomes in Pimpinella, which has limited systematic understanding of this genus. We assembled the complete chloroplast genomes of nine Pimpinella species from China using data generated from next generation sequencing (NGS). The chloroplast (cp) DNA used were standard double -stranded molecules, ranging from 146,432 base pairs (bp) (P. valleculosa) to 165,666 bp (P. purpurea) in length. The circular DNA contained a large single-copy (LSC) region, small single-copy (SSC) region, and pair of inverted repeats (IRs). The cp DNA of the nine species contained 82-93 protein-coding genes, 36-37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes, respectively. Four species (P. smithii, P. valleculosa, P. rhomboidea, and P. purpurea) exhibited striking distinctions in genome size, gene number, IR boundary, and sequence identity. We confirmed the non-monophyly of the Pimpinella species on the basis of the nine newly identified plastomes. The distant relationship between the above-mentioned four Pimpinella species and Pimpinelleae was indicated with high support values. Our study provides a foundation for future in-depth phylogenetic and taxonomic studies of genus Pimpinella.
摘要:
Arsenic (As) and cadmium (Cd) often coexist in paddy soils. Nano-ferrihydrite colloidal particles (NFPs) are ubiquitous at redox active interfaces of the paddy system and are well-known to play a critical role in controlling the solubility and bio-availability of As and Cd. However, the mutual interaction between As and Cd on NFPs remains elusive. Herein, batch experiments and in-situ spectroscopic techniques were used to investigate the effects of the interaction pattern (sequential reaction) of Cd(II) and As(V) on their respective adsorption on the surfaces of NFPs. Two scenarios were designed: Cd(II) pre-saturated NFPs and As(V) pre-saturated NFPs. Adsorption of Cd(II) was increased by 1.67, 4.08, and 5.21 times in As(V)-saturated NFPs, but only by 1.05, 1.11, and 1.15 times for As(V) in Cd(II)-saturated NFPs. Further, we determined the pH-dependent mutually beneficial cooperation pathways as mediated by the surface of NFPs. At lower pH (5), As(V) tended to promote Cd(II) adsorption, whereas Cd(II) tended to enhance As(V) adsorption at higher pH (> 5.5). X-ray photoelectron spectroscopy (XPS) indicated that both pre-saturated Cd(II) and As(V) altered the local coordination environment of their counterpart ions. Furthermore, results from in-situ attenuated total reflection-Fourier-transform infrared spectroscopy (ATR-FTIR) and second derivative peak shape fitting revealed two types of ternary surface complexes, namely Cd(II)-bridged and As(V)-bridged complexes, which were responsible for the distinct Cd(II) and As(V) co-adsorption behavior on the surface of NFPs under different conditions. These findings help us understand how co-presence Cd and As behave in an iron-rich geological setting and will aid in the development of related restoration technologies.
摘要:
Pseudomonas aeruginosa is a conditional Gram-negative pathogen that produces extracellular virulence factors that can lead to bloodstream invasion, severely harm tissues, and disseminate bacteria, ultimately leading to various diseases. In this study, lactic acid bacteria (LAB) with strong antagonistic ability against P. aeruginosa were screened, and the regulatory mechanism of LAB against P. aeruginosa was evaluated. The results showed that the three selected LAB strains had strong inhibition ability on the growth, biofilm formation, and pyocyanin expression of P. aeruginosa and a promoting effect on the expression of autoinducer-2. Among them, Lactipantibacillus plantarum (Lp. plantarum) LPyang is capable of affecting the metabolic processes of P. aeruginosa by influencing metabolic substances, such as LysoPC, oxidized glutathione, betaine, etc. These results indicate that LPyang reduces the infectivity of P. aeruginosa through inhibition of its growth, biofilm formation, pyocyanin expression, and regulation of its metabolome. This study provides new insights into the antagonistic activity of Lp. plantarum LPyang against P. aeruginosa.
摘要:
Simple Summary Habituation to deterrent plant compounds has been found in generalist and specialist insect herbivores. The rate at which plant-feeding insects habituate and at which sensitivity of taste neurons detecting deterrents changes has not been compared among closely related species. The generalist Helicoverpa armigera (Hubner) and the specialist Helicoverpa assulta (Guenee) (Lepidoptera: Noctuidae) differ strongly in degree of host-plant specialism. Both species habituated to the alkaloid strychnine after dietary exposure; however, the specialist H. assulta displayed habituation to strychnine faster (at 48 h) than the generalist H. armigera (at 72 h). Electrophysiological recordings from taste sensilla on mouthparts revealed that a deterrent-sensitive neuron in the medial sensillum styloconicum of both species displayed significantly reduced sensitivity to the deterrent that coincided in time with the onset of habituation. Our findings show that physiological changes in taste neuron sensitivity coincide with habituation to plant compounds that are initially avoided. The two closely related moth species, Helicoverpa armigera and H. assulta differ strongly in their degree of host-plant specialism. In dual-choice leaf disk assays, caterpillars of the two species that had been reared on standard artificial diet were strongly deterred by the plant-derived alkaloid strychnine. However, caterpillars of both species reared on artificial diet containing strychnine from neonate to the 5th instar were insensitive to this compound. Fifth instar caterpillars of H. assulta and 4th or 5th instars of H. armigera not exposed to strychnine before were subjected to strychnine-containing diet for 24 h, 36 h, 48 h, or 72 h. Whereas H. assulta displayed habituation to strychnine after 48 h, it took until 72 h for H. armigera to become habituated. Electrophysiological tests revealed that a deterrent-sensitive neuron in the medial sensillum styloconicum of both species displayed significantly reduced sensitivity to strychnine that correlated with the onset of habituation. We conclude that the specialist H. assulta habituated faster to strychnine than the generalist H. armigera and hypothesis that desensitization of deterrent-sensitive neurons contributed to habituation.
通讯机构:
[Li, F.] K;Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Science, Changsha, Hunan, China
期刊:
Annals of Medicine,2022年54(1):2785-2795 ISSN:0785-3890
通讯作者:
Fu, D.;Cao, Y.-F.;Ma, Y.-S.
作者机构:
[Diao, Xun; Li, Sha; Yu, Xue-Chen; Fan, Qi-Yu; Liu, Ji-Bin] Nantong Univ, Affiliated Tumor Hosp, Inst Oncol, Nantong, Peoples R China.;[Yu, Xue-Chen] Macalester Coll, Dept Math Stat & Comp Sci, St Paul, MN USA.;[Tang, Qing-Hai] Hengyang Normal Univ, Hunan Key Lab Conservat & Utilizat Biol Resource, Hengyang, Peoples R China.;[Tang, Qing-Hai] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang, Peoples R China.;[Tang, Xiao-Mei; Fu, Da; Huang, Zhong-Yan] Shanghai Jiao Tong Univ, Ruijin Hosp, Gen Surg, Sch Med, Shanghai, Peoples R China.
通讯机构:
[Yong-Feng Cao] D;[Yu-Shui Ma] C;[Da Fu] G;Department of Oncology, Affiliated Tumor Hospital of Nantong University, Nantong, China<&wdkj&>Cancer Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China<&wdkj&>General Surgery, Ruijin Hospital & Institute of Pancreatic Diseases, Shanghai Jiaotong University School of Medicine, Shanghai, China<&wdkj&>Central Laboratory for Medical Research, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China
期刊:
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy,2022年278:121290 ISSN:1386-1425
通讯作者:
Gu, B.
作者机构:
[Gu, Biao] Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China. Electronic address: biaogu@hynu.edu.cn;[Liu, Mengqin; Ye, Xinrong] Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China;[Long, Jiumei] College of Life Sciences and Environment, Hengyang Normal University, Hengyang 421008, PR China;[Xu, Zhifeng] Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, PR China. Electronic address: xuzhifenghwl@163.com;[Shen, Youming] College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China. Electronic address: ymshen79@huas.edu.cn
通讯机构:
Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, China
期刊:
Frontiers in Plant Science,2022年13:822374 ISSN:1664-462X
通讯作者:
Peng, Daiyin;Xing, SH;Peng, DY
作者机构:
[Xing, SH; Yao, Liang; Ye, Mengjuan; Xing, Shihai; Yao, Xiaoyan; Wang, Zhaojian; Wu, Jing; Zhang, Jing; Yu, Nianjun; Peng, Daiyin; Meng, Fei; Peng, DY] Anhui Univ Chinese Med, Coll Pharm, Hefei, Peoples R China.;[Xing, SH; Xing, Shihai; Wu, Jing; Yu, Nianjun; Peng, Daiyin; Peng, DY] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA.;[Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang, Peoples R China.;[Wang, Longhai] Anhui Univ Chinese Med, Sch Integrated Chinese & w Med, Hefei, Peoples R China.
通讯机构:
[Xing, SH ; Peng, DY] A;[Peng, DY ] S;Anhui Univ Chinese Med, Coll Pharm, Hefei, Peoples R China.;Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei, Peoples R China.;Synerget Innovat Ctr Anhui Authent Chinese Med Qu, Hefei, Peoples R China.
期刊:
Pakistan Journal of Botany,2022年54(5):1859-1864 ISSN:0556-3321
通讯作者:
Wang, Z.
作者机构:
[Wang, Zhixin; Cao, Limin; Liu, Jianhui] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Peoples R China.
通讯机构:
Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences and Environment, Hengyang Normal University, Hengyang, China