作者机构：
[Yang, Qingshan; Wang, Shuting; Liu, Juan; Xing, SH; Peng, Daiyin; Xing, Shihai; Xu, Shaowei; Meng, Fei] Anhui Univ Chinese Med, Coll Pharm, Hefei 230012, Peoples R China.;[Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Hunan, Peoples R China.;[Xing, SH; Peng, Daiyin; Xing, Shihai; Meng, Fei] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;[Sun, Zongping] Fuyang Normal Univ, Engn Technol Res Ctr Antiaging, Chinese Herbal Med, Fuyang 236037, Peoples R China.;[Xing, SH; Xing, Shihai; Chen, Na] Hefei Comprehens Natl Sci Ctr, Joint Res Ctr Chinese Herbal Med Anhui IHM, Bozhou 236814, Peoples R China.
通讯机构：
[Liu, J; Xing, SH ] A;Anhui Univ Chinese Med, Coll Pharm, Hefei 230012, Peoples R China.;Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;Hefei Comprehens Natl Sci Ctr, Joint Res Ctr Chinese Herbal Med Anhui IHM, Bozhou 236814, Peoples R China.
摘要：
In recent years, it was found that lysine malonylation modification can affect biological metabolism and play an important role in plant life activities. Platycodon grandiflorus, an economic crop and medicinal plant, had no reports on malonylation in the related literature. This study qualitatively introduces lysine malonylation in P. grandiflorus. A total of 888 lysine malonylation-modified proteins in P. grandiflorus were identified, with a total of 1755 modification sites. According to the functional annotation, malonylated proteins were closely related to catalysis, binding, and other reactions. Subcellular localization showed that related proteins were enriched in chloroplasts, cytoplasm, and nuclei, indicating that this modification could regulate various metabolic processes. Motif analysis showed the enrichment of Alanine (A), Cysteine (C), Glycine (G), and Valine (V) amino acids surrounding malonylated lysine residues. Metabolic pathway and protein-protein interaction network analyses suggested these modifications are mainly involved in plant photosynthesis. Moreover, malonylated proteins are also involved in stress and defense responses. This study shows that lysine malonylation can affect a variety of biological processes and metabolic pathways, and the contents are reported for the first time in P. grandiflorus, which can provide important information for further research on P. grandiflorus and lysine malonylation's role in environment stress, photosynthesis, and secondary metabolites enrichment.
摘要：
Soil bacteria, as integral components of the soil microbial community, play pivotal roles in biogeochemical cycles and ecosystem functions in boreal forests. The altitudinal patterns of soil bacteria have been widely reported, but their community assembly is uncertain. Here, we investigated the soil bacterial community attributes (diversity, taxonomic and functional composition, and bacterial interactions) and ecological processes associated with community assembly on Mt. Oakley in the northern Greater Khingan Mountains via Illumina MiSeq sequencing and functional annotation tools. The alpha diversity indices of the soil bacteria exhibited a progressively decreasing trend with increasing altitude across seasons. The relative abundance of the dominant bacterial taxa was more sensitive to altitude than to season. More complex (more nodes and links) bacterial interactions were detected at the lowest and highest altitudinal sites, as well as in September. The variations in the taxonomic and functional compositions of the soil bacteria induced by altitude were mainly driven by the variations in soil pH and extracellular enzyme activities. Stochastic (dispersal limitation and drift) processes largely controlled the soil bacterial community assemblages across spatiotemporal scales. The community assemblages of soil bacteria were affected by altitude-induced changes in the taxonomic composition and functional groups. Collectively, our results have significant implications for understanding bacterial biogeography and community assembly mechanisms along altitudinal gradients in boreal forest ecosystems.
通讯机构：
[Wei Liu; Xuexiao Zou] C;[Zhuqing Zhang] V;College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>Longping Branch, Graduate School of Hunan University, Changsha, Hunan 410125, Chin<&wdkj&>College of Life Sciences and Environment, Hengyang Normal University, Hengyang, Henan 421008, China<&wdkj&>Vegetable Institution of Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China
作者机构：
[Wang, Zhixin; Cao, Limin; Liu, Jianhui] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang, Hunan, Peoples R China.;[He, Xingjin] Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ, Chengdu, Sichuan, Peoples R China.
关键词：
chloroplast DNA;circular DNA;leucine;nadh dehydrogenase subunit 2;plant protein;protein tic 214;ribosomal protein l14;ribosomal protein l16;ribosomal protein l2;ribosomal protein l22;ribosomal protein l23;ribosomal protein l36;ribosomal protein s12;ribosomal protein s3;ribosomal protein s7;ribosomal protein s8;ribosomal protein S9;ribosome RNA;transfer RNA;translation initiation factor if1;unclassified drug;ycf2 protein;Article;China;chloroplast genome;codon usage;complete plastomes;DNA base composition;DNA extraction;DNA sequence;gene number;genetic distance;genome size;high throughput sequencing;inverted repeat boundary;molecular genetic phenomena and functions;nonhuman;phylogeny;Pimpinella;Pimpinella candolleana;Pimpinella diversifolia;Pimpinella purpurea;Pimpinella rhomboidea;Pimpinella rubescens;Pimpinella scaberula;Pimpinella smithii;Pimpinella thellungiana;Pimpinella valleculosa;plant genome;RNA editing site
摘要：
Pimpinella L. is one of the large genera in the Apiaceae family. In a previous study, the molecular phylogenies of Pimpinella were explored using nuclear ribosomal DNA internal transcribed spacers (ITS) and several chloroplast DNA segments. There have been few studies conducted on chloroplast genomes in Pimpinella, which has limited systematic understanding of this genus. We assembled the complete chloroplast genomes of nine Pimpinella species from China using data generated from next generation sequencing (NGS). The chloroplast (cp) DNA used were standard double-stranded molecules, ranging from 146,432 base pairs (bp) (P. valleculosa) to 165,666 bp (P. purpurea) in length. The circular DNA contained a large single-copy (LSC) region, small single-copy (SSC) region, and pair of inverted repeats (IRs). The cp DNA of the nine species contained 82–93 protein-coding genes, 36–37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes, respectively. Four species (P. smithii, P. valleculosa, P. rhomboidea, and P. purpurea) exhibited striking distinctions in genome size, gene number, IR boundary, and sequence identity. We confirmed the nonmonophyly of the Pimpinella species on the basis of the nine newly identified plastomes. The distant relationship between the above-mentioned four Pimpinella species and Pimpinelleae was indicated with high support values. Our study provides a foundation for future in-depth phylogenetic and taxonomic studies of genus Pimpinella. Copyright 2023 Wang et al.
期刊：
Journal of Hazardous Materials,2023年447:130755 ISSN：0304-3894
通讯作者：
Lei, Ming
作者机构：
[Huang, Yayuan; Mensah, Caleb Oppong; Tie, Boqing; Li, Bingyu; Wei, Dongning; Lei, Ming; Zhou, Yimin; Jing, Miaomiao] Hunan Agr Univ, Coll Resource & Environm, Changsha 410128, Peoples R China.;[Huang, Yayuan; Mensah, Caleb Oppong; Tie, Boqing; Li, Bingyu; Lei, Ming; Zhou, Yimin; Jing, Miaomiao] Hunan Engn & Technol Res Ctr Irrigat Water Purific, Changsha 410128, Peoples R China.;[Long, Jiumei] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Peoples R China.;[Lei, Ming] 1st Nongda Rd, Changsha, Peoples R China.
通讯机构：
[Ming Lei] C;College of Resource and Environment, Hunan Agricultural University, Changsha 410128, PR China<&wdkj&>Hunan Engineering and Technology Research Center for Irrigation Water Purification, Changsha 410128, PR China
摘要：
Pseudomonas aeruginosa is a conditional Gram-negative pathogen that produces extracellular virulence factors that can lead to bloodstream invasion, severely harm tissues, and disseminate bacteria, ultimately leading to various diseases. In this study, lactic acid bacteria (LAB) with strong antagonistic ability against P. aeruginosa were screened, and the regulatory mechanism of LAB against P. aeruginosa was evaluated. The results showed that the three selected LAB strains had strong inhibition ability on the growth, biofilm formation, and pyocyanin expression of P. aeruginosa and a promoting effect on the expression of autoinducer-2. Among them, Lactipantibacillus plantarum (Lp. plantarum) LPyang is capable of affecting the metabolic processes of P. aeruginosa by influencing metabolic substances, such as LysoPC, oxidized glutathione, betaine, etc. These results indicate that LPyang reduces the infectivity of P. aeruginosa through inhibition of its growth, biofilm formation, pyocyanin expression, and regulation of its metabolome. This study provides new insights into the antagonistic activity of Lp. plantarum LPyang against P. aeruginosa.
摘要：
Simple Summary Habituation to deterrent plant compounds has been found in generalist and specialist insect herbivores. The rate at which plant-feeding insects habituate and at which sensitivity of taste neurons detecting deterrents changes has not been compared among closely related species. The generalist Helicoverpa armigera (Hubner) and the specialist Helicoverpa assulta (Guenee) (Lepidoptera: Noctuidae) differ strongly in degree of host-plant specialism. Both species habituated to the alkaloid strychnine after dietary exposure; however, the specialist H. assulta displayed habituation to strychnine faster (at 48 h) than the generalist H. armigera (at 72 h). Electrophysiological recordings from taste sensilla on mouthparts revealed that a deterrent-sensitive neuron in the medial sensillum styloconicum of both species displayed significantly reduced sensitivity to the deterrent that coincided in time with the onset of habituation. Our findings show that physiological changes in taste neuron sensitivity coincide with habituation to plant compounds that are initially avoided. The two closely related moth species, Helicoverpa armigera and H. assulta differ strongly in their degree of host-plant specialism. In dual-choice leaf disk assays, caterpillars of the two species that had been reared on standard artificial diet were strongly deterred by the plant-derived alkaloid strychnine. However, caterpillars of both species reared on artificial diet containing strychnine from neonate to the 5th instar were insensitive to this compound. Fifth instar caterpillars of H. assulta and 4th or 5th instars of H. armigera not exposed to strychnine before were subjected to strychnine-containing diet for 24 h, 36 h, 48 h, or 72 h. Whereas H. assulta displayed habituation to strychnine after 48 h, it took until 72 h for H. armigera to become habituated. Electrophysiological tests revealed that a deterrent-sensitive neuron in the medial sensillum styloconicum of both species displayed significantly reduced sensitivity to strychnine that correlated with the onset of habituation. We conclude that the specialist H. assulta habituated faster to strychnine than the generalist H. armigera and hypothesis that desensitization of deterrent-sensitive neurons contributed to habituation.
通讯机构：
[Fengna Li; Yulong Yin] K;Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Science, Changsha, Hunan 410125, China<&wdkj&>Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Hunan Normal University, Changsha, Hunan 410081, China<&wdkj&>Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Science, Changsha, Hunan 410125, China
摘要：
To study the effects of dietary energy level on the meat quality of different muscles in finishing pigs, 400 Xiangcun Black pigs (BW 1/4 79.55 & PLUSMN; 4.77 kg) were randomly assigned to 5 treatments with varied calculated digestive energy (DE) at 3,050, 3,100, 3,150, 3,200 and 3,250 kcal/kg, respectively. Each treatment had 8 replicates with 10 pigs per replicate. Meat quality, amino acid and fatty acid composition were tested in this study. No differences in average daily gain, average daily feed intake or feed-to-gain ratio (P > 0.05) were observed among dietary treatments. Glycogen concentrations of longissimus dorsi (LD) muscle in DE3150 was higher than those in other groups (P < 0.05). The crude fat concentration of biceps femoris (BF) muscle in DE3250 tended to be higher than that in DE3150 and DE3100 groups (P < 0.05). Pigs in DE3250 and DE3200 had higher fiber density and smaller cross-sectional area of BF muscle than those in DE3150 (P < 0.05). Pigs in DE3150 had the highest Cu concentration in LD muscle compared with those in DE3200, DE3250 (P < 0.05). The C16:1 proportion of LD muscle was lower (P < 0.01) and C20:1 was higher (P < 0.05) in DE3050 than that in the other dietary treatments. The C18:3n6 and C20:3n6 proportions of BF muscle in DE3150 were higher than those in DE 3050, DE3200 and DE3250 (P < 0.05). For LD muscle, mRNA ex-pressions of type I and IIa MyHC in group DE3150 were higher than other treatments (P < 0.01). The LD muscle in DE3150 expressed higher PPARd than in other groups (P < 0.01). Pigs in DE3100 expressed higher FOX1 than in DE3200 and DE3250 (P < 0.05). Sterol-regulatory element binding proteins (SREBPa) mRNA expression decreased linearly when dietary energy level increased in BF muscle (P < 0.01). In conclusion, a 200 kcal/kg decrease in digestible energy for 4 consecutive weeks did not affect growth performance of Xiangcun Black pigs. Furthermore, LD and BF muscle respond differently to dietary energy level, and meat quality was improved by the medium energy level during the finishing phase. (c) 2022 Chinese Association of Animal Science and Veterinary Medicine. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
摘要：
Nitric oxide (NO), an essential biological messenger molecule, participates in various physiological and pathological processes. The sensitive and specific detection of NO is of great significance for understanding the biological function of NO. Here, we synthesized a fluorescent probe (Rho-NO) for highly selective detection of NO both in vitro and in vivo. The high selectivity of Rho-NO is attributed to the fact that NO is easily replaced by electron donor amino group to form N-nitrosation products, causing rhodamine spiro ring open and fluorescence emit. Rho-NO showed a good linear response to NO (0-100 mu M) with a low detection limit (0.06 mu M). Importantly, it exhibited excellent specificity for NO detection in human serum and was also applied for imaging NO in living cells and inflammatory model of zebrafish. This work proves the potential of Rho-NO in pathological research and disease diagnosis. (C) 2021 Elsevier B.V. All rights reserved.
期刊：
FRONTIERS IN PLANT SCIENCE,2022年13:822374 ISSN：1664-462X
通讯作者：
Peng, Daiyin;Xing, SH;Peng, DY
作者机构：
[Xing, SH; Yao, Liang; Ye, Mengjuan; Xing, Shihai; Yao, Xiaoyan; Wang, Zhaojian; Wu, Jing; Zhang, Jing; Yu, Nianjun; Peng, Daiyin; Meng, Fei; Peng, DY] Anhui Univ Chinese Med, Coll Pharm, Hefei, Peoples R China.;[Xing, SH; Xing, Shihai; Wu, Jing; Yu, Nianjun; Peng, Daiyin; Peng, DY] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA.;[Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang, Peoples R China.;[Wang, Longhai] Anhui Univ Chinese Med, Sch Integrated Chinese & w Med, Hefei, Peoples R China.
通讯机构：
[Xing, SH ; Peng, DY] A;[Peng, DY ] S;Anhui Univ Chinese Med, Coll Pharm, Hefei, Peoples R China.;Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei, Peoples R China.;Synerget Innovat Ctr Anhui Authent Chinese Med Qu, Hefei, Peoples R China.
期刊：
PAKISTAN JOURNAL OF BOTANY,2022年54(5):1859-1864 ISSN：0556-3321
通讯作者：
Wang, Z.
作者机构：
[Wang, Zhixin; Cao, Limin; Liu, Jianhui] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Peoples R China.
通讯机构：
Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences and Environment, Hengyang Normal University, Hengyang, China
