期刊：
MATHEMATICAL BIOSCIENCES AND ENGINEERING,2024年21(2):2344-2365 ISSN：1547-1063
作者机构：
[Haihua Zhou] School of Mathematics and Statistics, Hengyang Normal University, Hengyang 421010, China;[Yaxin Liu; Zejia Wang; Huijuan Song] School of Mathematics and Statistics, Jiangxi Normal University, Nanchang 330022, China
摘要：
<jats:p xml:lang="fr"><abstract><p>This paper was concerned with a free boundary problem modeling the growth of tumor cord with a time delay in cell proliferation, in which the cell location was incorporated, the domain was bounded in $ \mathbb{R}^2 $, and its boundary included two disjoint closed curves, one fixed and the other moving and a priori unknown. A parameter $ \mu $ represents the aggressiveness of the tumor. We proved that there exists a unique radially symmetric stationary solution for sufficiently small time delay, and this stationary solution is linearly stable under the nonradially symmetric perturbations for any $ \mu &gt; 0 $. Moreover, adding the time delay in the model leads to a larger stationary tumor. If the tumor aggressiveness parameter is bigger, the time delay has a greater effect on the size of the stationary tumor, but it has no effect on the stability of the stationary solution.</p></abstract></jats:p>
通讯机构：
[Wei Liu; Xuexiao Zou] C;[Zhuqing Zhang] V;College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>Longping Branch, Graduate School of Hunan University, Changsha, Hunan 410125, Chin<&wdkj&>College of Life Sciences and Environment, Hengyang Normal University, Hengyang, Henan 421008, China<&wdkj&>Vegetable Institution of Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China
期刊：
JOURNAL OF MEDICINAL CHEMISTRY,2023年66(6):4045-4058 ISSN：0022-2623
通讯作者：
Cui-Yun Yu<&wdkj&>Hua Wei
作者机构：
[Liu, Hongbing; Lu, Linyin; Wei, Hua; Liu, Ying; Liu, Li; Liu, Xuyue; Li, Shuang; He, Lifang; Wang, Dun; Zhou, Zongtao; He, Dongxiu; Yu, Cui-Yun] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Liu, Hongbing; Lu, Linyin; Wei, Hua; Liu, Ying; Liu, Li; Liu, Xuyue; Li, Shuang; Wang, Dun; Zhou, Zongtao; He, Dongxiu; Yu, Cui-Yun] Univ South China, Sch Pharmaceut Sci, Hengyang 421001, Peoples R China.;[He, Lifang] Hengyang Normal Univ, Coll Life Sci, Hengyang 421001, Peoples R China.
通讯机构：
[Cui-Yun Yu; Hua Wei] H;Hengyang Medical School, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study & School of Pharmaceutical Science, University of South China, Hengyang 421001, China
摘要：
Scalable production of a clinically translatable formulation with enhanced therapeutic efficacy against cisplatin-resistant tumors without the use of any clinically unapproved reagents and additional manipulation remains a challenge. For this purpose, we report herein the construction of TPP-Pt-acetal-CA based on all commercially available, clinically approved reagents consisting of a cinnamaldehyde (CA) unit for reactive oxygen species generation, a mitochondrially targeted triphenylphosphonium (TPP)-modified Pt(IV) moiety for mitochondrial dysfunction, and an intracellular acidic pH-cleavable acetal link between these two moieties. The resulting self-assembled, stabilized TPP-Pt-acetal-CA nanoparticles mediated an IC50 value approximately 6-fold lower than that of cisplatin in A549/DDP cells and a tumor weight reduction 3.6-fold greater than that of cisplatin in A549/DDP tumor-bearing BALB/c mice with insignificant systematic toxicity due to the synergistic mitochondrial dysfunction and markedly amplified oxidative stress. Therefore, this study presents the first example of a clinically translatable Pt(IV) prodrug with enhanced efficiency for synergistically reversing drug resistance.
作者机构：
[Liu, Yuan; Tang, Yaqin; Wu, Mengqi; Liu, Xiaohua; Huang, Shuaiqin; Hou, Shengjie; Li, Jing; Jiang, Liping] Cent South Univ, Xiangya Sch Med, Dept Parasitol, Changsha 410013, Peoples R China.;[Tang, Xing] Hengyang Normal Univ, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Peoples R China.;[Deng, Meichun] Cent South Univ, Sch Life Sci, Dept Biochem & Mol Biol, Changsha 410013, Peoples R China.;[Jiang, Liping] Cent South Univ, Xiangya Sch Med, China Africa Res Ctr Infect Dis, Changsha 410013, Peoples R China.
通讯机构：
[Liping Jiang] C;China-Africa Research Center of Infectious Diseases, Xiangya School of Medicine, Central South University, Changsha 410013, China<&wdkj&>Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410013, China<&wdkj&>Author to whom correspondence should be addressed.
通讯机构：
[Shiqing Tang] C;College of Physics and Electronic Engineering, Hengyang Normal University, Hengyang 421002, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
Berry phase;Dicke quantum phase transition;two impurity qubits;dispersive interaction;X-type state
摘要：
In this paper, we investigate the effect of the Dicke quantum phase transition on the Berry phase of the two impurity qubits. The two impurity qubits only have dispersive interactions with the optical field of the Dicke quantum system. Therefore, the two impurity qubits do not affect the ground state energy of the Dicke Hamiltonian. We find that the Berry phase of the two impurity qubits has a sudden change at the Dicke quantum phase transition point. Therefore, the Berry phase of the two impurity qubits can be used as a phase transition signal for the Dicke quantum phase transition. In addition, the two impurity qubits change differently near the phase transition point at different times. We explain the reason for the different variations by studying the variation of the Berry phase of the two impurity qubits with the phase transition parameters and time. Finally, we investigated the variation of the Berry phases of the two impurity qubits with their initial conditions, and we found that their Berry phases also have abrupt changes with the initial conditions. Since the Dicke quantum phase transition is already experimentally executable, the research in this paper helps to provide a means for manipulating the Berry phase of the two impurity qubits.
作者机构：
[Zhang, Zhongbo] Hunan Weather Modificat Off, Changsha 410118, Peoples R China.;[Zhang, Zhongbo] Hunan Key Lab Meteorol Disaster Prevent & Mitigat, Changsha 410118, Peoples R China.;[Liu, Yuan; Tang, Wenwen; Fu, Jing; Fang, Xiaohong; Zhang, Haibo] Hengyang Normal Univ, Coll Geog & Tourism, Hengyang 421002, Peoples R China.;[Fu, Jing] Int Ctr Space Technol Nat & Cultural Heritage HIS, Hengyang Base 421002, Hengyang, Peoples R China.;[Fu, Jing] Hunan Normal Univ, Hunan Key Lab Geospatial Big Data Min & Applicat, Changsha 410081, Peoples R China.
通讯机构：
[Jing Fu] C;College of Geography and Tourism, Hengyang Normal University, Hengyang 421002, China<&wdkj&>International Centre on Space Technologies for Natural and Cultural Heritage (HIST) under the Auspices of UNESCO, Hengyang Base, Hengyang 421002, China<&wdkj&>Hunan Key Laboratory of Geospatial Big Data Mining and Application, Hunan Normal University, Changsha 410081, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
It is important to reveal the spatial and temporal variations of drought and evaluate the alleviating effects of artificial precipitation on drought severity, as it will contribute immensely to the formulation of drought prevention and mitigation measures and the provision of guidance to artificial precipitation enhancement operation. Based on the monthly precipitation data of 28 meteorological stations in Hengyang-Shaoyang Drought Corridor (HSDC) from 1960 to 2019, the standardized precipitation index (SPI) at multiple time scales were calculated to estimate drought frequency, drought station ratio, and drought intensity. Then the spatiotemporal variations of drought in the study area were unveiled, and the effects of artificial precipitation enhancement were evaluated in line with the relevant data from 2005 to 2019. The results show that at the annual scale, drought occurred in 3/4 of past sixty years in the study area, where almost 1/3 of such years experienced area-wide droughts. Drought coverage in HSDC exhibited a decreasing trend, but drought intensity, as well as the number of area-wide droughts and regional droughts showed an increasing one. Mild and moderate droughts occurred in an extensive part of the HSDC, whereas severe and extreme droughts were mainly found in a few stations. At the seasonal scale, winter drought occurred most frequently, followed by summer and autumn droughts, while spring drought events had the lowest frequency. Overall, drought is more serious in spring, autumn, and winter, but less severe in summer; although drought intensity decreased slightly in summer, both its intensity and coverage showed an increasing trend in other seasons. At the monthly scale, the ratio of positive to negative SPI values in HSDC was basically balanced in the past six decades, exhibiting no distinct variation characteristics. In addition, artificial precipitation enhancement effectively eased monthly and even seasonal drought in HSDC. These findings, which fully reflect the characteristics of drought in the study area, can also raise awareness of the contribution that artificial precipitation could make to drought mitigation, which in turn will contribute to the formulation of appropriate strategies for climate change mitigation and adaptation.
期刊：
FRONTIERS IN PLANT SCIENCE,2022年13:937392 ISSN：1664-462X
通讯作者：
Xing, S.;Peng, D.
作者机构：
[Wu, Liping; Peng, Daiyin; Xing, Shihai; Su, Xinglong; Meng, Fei; Wang, Zhaojian] Anhui Univ Chinese Med, Coll Pharm, Hefei, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, St Paul, MN USA.;[Huang, Huizhen; Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang, Peoples R China.;[Liu, Yingying] Anhui Univ Chinese Med, Coll Humanities & Int Educ Exchange, Hefei, Peoples R China.;[Wang, Longhai] Anhui Univ Chinese Med, Sch Integrated Chinese & Western Med, Hefei, Peoples R China.
通讯机构：
[Peng, D.; Xing, S.] C;College of Pharmacy, China
关键词：
Dendrobium huoshanense;greenhouse planting;phosphoproteome;proteome;stone planting under the forest
摘要：
The highly esteemed Chinese herb, Dendrobium huoshanense, whose major metabolites are polysaccharides and alkaloids, is on the verge of extinction. The stone planting under the forest (SPUF) and greenhouse planting (GP) of D. huoshanense are two different cultivation methods of pharmaceutical Dendrobium with significantly differences in morphology, metabolites content and composition, and medication efficacy. Here, we conducted proteomics and phosphoproteomics analyses to reveal differences in molecular mechanisms between SPUF and GP. We identified 237 differentially expressed proteins (DEPs) between the two proteomes, and 291 modification sites belonging to 215 phosphoproteins with a phosphorylation level significantly changed (PLSC) were observed. GO, KEGG pathway, protein domain, and cluster analyses revealed that these DEPs were mainly localized in the chloroplast; involved in processes such as posttranslational modification, carbohydrate transport and metabolism, and secondary metabolite biosynthesis; and enriched in pathways mainly including linoleic acid metabolism, plant-pathogen interactions, and phenylpropanoid, cutin, suberin, and wax biosynthesis. PLSC phosphoproteins were mainly located in the chloroplast, and highly enriched in responses to different stresses and signal transduction mechanisms through protein kinase and phosphotransferase activities. Significant differences between SPUF and GP were observed by mapping the DEPs and phosphorylated proteins to photosynthesis and polysaccharide and alkaloid biosynthesis pathways. Phosphorylation characteristics and kinase categories in D. huoshanense were also clarified in this study. We analyzed different molecular mechanisms between SPUF and GP at proteomic and phosphoproteomic levels, providing valuable information for the development and utilization of D. huoshanense.
摘要：
<jats:p>Platycodin D (PD) is a deglycosylated triterpene saponin with much higher pharmacological activity than glycosylated platycoside E (PE). Extensive studies <jats:italic>in vitro</jats:italic> showed that the transformation of platycoside E to platycodin D can be achieved using β-glucosidase extracted from several bacteria. However, whether similar enzymes in <jats:italic>Platycodon grandiflorus</jats:italic> could convert platycoside E to platycodin D, as well as the molecular mechanism underlying the deglycosylation process of platycodon E, remain unclear. Here, we identified a β-glucosidase in <jats:italic>P. grandiflorus</jats:italic> from our previous RNA-seq analysis, with a full-length cDNA of 1,488 bp encoding 495 amino acids. Bioinformatics and phylogenetic analyses showed that β-glucosidases in <jats:italic>P. grandiflorus</jats:italic> have high homology with other plant β-glucosidases. Subcellular localization showed that there is no subcellular preference for its encoding gene. β-glucosidase was successfully expressed as 6 × His-tagged fusion protein in <jats:italic>Escherichia coli</jats:italic> BL21 (DE3). Western blot analysis yielded a recombinant protein of approximately 68 kDa. <jats:italic>In vitro</jats:italic> enzymatic reactions determined that β-glucosidase was functional and could convert PE to PD. RT-qPCR analysis showed that the expression level of β-glucosidase was higher at night than during the day, with the highest expression level between 9:00 and 12:00 at night. Analysis of the promoter sequence showed many light-responsive cis-acting elements, suggesting that the light might regulate the gene. The results will contribute to the further study of the biosynthesis and metabolism regulation of triterpenoid saponins in <jats:italic>P. grandiflorus</jats:italic>.</jats:p>
期刊：
Environmental Science and Pollution Research,2022年29(30):46161-46173 ISSN：0944-1344
通讯作者：
Tang, WQ
作者机构：
[Liu, Yan; Liu, Xing; Wang, Shuzhan; Wang, Zefen; Tang, Wenqing; Chen, Zhen; Yi, Nengzhong; Chen, Jun; Zhou, Qianyi; Zeng, Rongying] Hengyang Normal Univ, Coll Chem & Mat Sci, Key Lab Funct Organometall Mat Coll Hunan Prov, Hunan Engn Res Ctr Monitoring & Treatment Heavy M, Hengyang 421001, Peoples R China.;[Chen, Jun] Changde Xinfurong Environm Protect Co Ltd, Changde, Peoples R China.
通讯机构：
[Tang, WQ ] H;Hengyang Normal Univ, Coll Chem & Mat Sci, Key Lab Funct Organometall Mat Coll Hunan Prov, Hunan Engn Res Ctr Monitoring & Treatment Heavy M, Hengyang 421001, Peoples R China.
期刊：
Journal of Ethnopharmacology,2022年293:115329 ISSN：0378-8741
通讯作者：
Shihai Xing<&wdkj&>Weimin Jiang
作者机构：
[Guo, Xiaohu; Xing, Shihai; Yao, Xiaoyan; Cao, Mengyang; Wu, Jing; Peng, Daiyin; Peng, Huasheng; Meng, Fei] Anhui Univ Chinese Med, Coll Pharm, Hefei 230012, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, Minneapolis, MN 55108 USA.;[Jiang, Weimin; Li, Yan] Hengyang Normal Univ, Coll Life Sci & Environm, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Peoples R China.;[Qu, Changqing] Fuyang Normal Univ, Engn Technol Res Ctr Antiaging, Chinese Herbal Med, Fuyang 236037, Peoples R China.;[Liu, Yingying] Anhui Univ Chinese Med, Coll Humanities & Int Educ Exchange, Hefei 230012, Peoples R China.
通讯机构：
[Shihai Xing] C;[Weimin Jiang] H;College of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China<&wdkj&>Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Hefei, 230038, China<&wdkj&>Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei, 230012, China<&wdkj&>Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences and Environment, Hengyang Normal University, Hengyang, 421008, China
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Salvia miltiorrhiza Bunge is a bulk medicinal material used in traditional Chinese medicine, that can cure cardiovascular diseases, neurasthenia, and other conditions. Sweating is a frequently used method of processing S. miltiorrhiza for medical applications. We previously demonstrated changes to the metabolic profile of linoleic acid, glyoxylate, and dicarboxylate after Sweating. However, this alteration has not been explained at the molecular level. MATERIALS AND METHODS: Fresh roots of Salvia miltiorrhiza Bunge were treated by the Sweating processing, and then the tandem mass tag technique was used to compare the proteome difference between Sweating S. miltiorrhiza and non-Sweating S. miltiorrhiza. RESULTS: We identified a total of 850 differentially expressed proteins after Sweating treatment in S. miltiorrhiza, including 529 upregulated proteins and 321 downregulated proteins. GO enrichment analysis indicated that these differentially expressed proteins are involved in external encapsulating structure, cell wall, oxidoreductase activity, ligase activity, and others. Further analysis showed that CYP450, the pathogenesis-related protein Bet v 1 family, and the peroxidase domain were the major protein domains. KEGG enrichment identified 18 pathways, of which phenylpropanoid biosynthesis is the most important one related to the metabolite profile and is the principal chemical component of S. miltiorrhiza. CONCLUSION: This study addressed potential molecular mechanisms in S. miltiorrhiza after Sweating, and the findings provide reasons for the changes in biochemical properties and metabolites changes which might cause pharmacological variation at the proteome level.
摘要：
It is found that the growth of Dendrobium huoshanense was dependent on Fe3O4, while the bioavailability of plants to ordinary Fe3O4 was low on the earth. In order to improve the growth, quality and yield of D. huoshanense, we used Fe3O4 NPs (100 or 200 mg/L) that was easily absorbed by plants as nano-fertilizer to hydroponically treat seedlings of D. huoshanense for 3 weeks. Fe3O4 NPs induced not only earlier flowering and increased sugar content and photosynthesis, but also stressed to plants, increased MDA content and related antioxidant enzymes activities. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) revealed that Fe3O4 NPs caused a significant accumulation of Fe and some other nutrient elements (Mn, Co, B, Mo) in stems of D. huoshanense. Metabolomics revealed that the metabolites were reprogrammed in D. huoshanense when under Fe3O4 NPs exposure. Fe3O4 NPs inhibited antioxidant defense-related pathways, demonstrating that Fe3O4 NPs have antioxidant capacity to protect D. huoshanense from damage. As the first study associating Fe3O4 NPs with the quality of D. huoshanense, it provided vital insights into the molecular mechanisms of how D. huoshanense responds to Fe3O4 NPs, ensuring the reasonable use of Fe3O4 NPs as nano-fertilizer.
作者机构：
[Wu, Liping; Yu, Nianjun; Peng, Daiyin; Xing, Shihai; Su, Xinglong; Cao, Mengyang; Wang, Zhaojian] Anhui Univ Chinese Med, Coll Pharm, Hefei 230012, Peoples R China.;[Peng, Daiyin; Xing, Shihai; Wang, Zhaojian] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;[Jiang, Weimin] Hengyang Normal Univ, Hunan Key Lab Conservat & Utilizat Biol Resources, Hengyang 421008, Peoples R China.;[Liu, Yingying] Anhui Univ Chinese Med, Coll Humanities & Int Educ Exchange, Hefei 230012, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, Minneapolis, MN 55108 USA.
通讯机构：
[Shihai Xing; Daiyin Peng] C;College of Pharmacy, Anhui University of Chinese Medicine, Hefei, China<&wdkj&>Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, China<&wdkj&>Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Hefei, China<&wdkj&>College of Pharmacy, Anhui University of Chinese Medicine, Hefei, China<&wdkj&>Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, China<&wdkj&>Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei, China
摘要：
<jats:title>Abstract</jats:title><jats:sec>
<jats:title>Background</jats:title>
<jats:p><jats:italic>Dendrobium officinale</jats:italic>, an endangered Chinese herb, possesses extensive therapeutic effects and contains bioactive ingredients such as major polysaccharides, alkaloids, and minimal flavonoids. We first obtained the protocorm-like bodies (PLBs) of this plant through tissue culture in order to determine the distribution of the main secondary metabolites in each organelle and the PLBs. We then analyzed the correlation between gene expression level from comparative transcriptome sequencing and metabolite content in different organs to identify putative genes encoding enzymes involved in the biosynthesis of polysaccharides, alkaloids, and flavonoids.</jats:p>
</jats:sec><jats:sec>
<jats:title>Results</jats:title>
<jats:p>We used seeds as explants for protocorm induction and PLB propagation of <jats:italic>D. officinale</jats:italic>. The optimal medium formula for PLB propagation was 1/2 MS + α-NAA 0.5 mg·L<jats:sup>− 1</jats:sup> + 6-BA 1.0 mg·L<jats:sup>− 1</jats:sup> + 2, 4-D 1.5–2.0 mg·L<jats:sup>− 1</jats:sup> + potato juice 100 g·L<jats:sup>− 1</jats:sup>. Stems, PLBs and leaves of <jats:italic>D. officinale</jats:italic> had the highest content of polysaccharides, alkaloids and flavonoids, respectively. Naringenin was only produced in stem; however, PLBs with high alkaloid content can replace other organs producing alkaloids. The hot water extraction method outperformed the ultrasound-assisted extraction method for extracting polysaccharides from <jats:italic>D. officinale.</jats:italic> A comparative transcriptome analysis of PLBs and leaves of <jats:italic>D. officinale</jats:italic> revealed differential expression of genes encoding enzymes involved in polysaccharide, alkaloid and flavonoid biosynthetic pathways. Putative genes encoding enzymes involved in these biosynthetic pathways were identified. Notably, we identified genes encoding the alkaloid biosynthesis enzymes strictosidine β-D-Glucosidase, geissoschizine synthase and vinorine synthase in <jats:italic>D. officinale</jats:italic>.</jats:p>
</jats:sec><jats:sec>
<jats:title>Conclusions</jats:title>
<jats:p>The identification of candidate genes encoding enzymes involved in metabolite biosynthesis will help to explore and protect this endangered species and facilitate further analysis of the molecular mechanism of secondary metabolite biosynthesis in <jats:italic>D. officinale</jats:italic>.</jats:p>
</jats:sec>
期刊：
Bioprocess and Biosystems Engineering,2020年43(4):701-710 ISSN：1615-7591
通讯作者：
Sun, Weifeng
作者机构：
[Chen, Xiaohua; Sun, Weifeng; Liu, Yi] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Peoples R China.;[Wang, Li; Lin, Lu; Wu, Lunjie; Ding, Wenwu; Che, Zhenming; Wu, Yuanming] Xihua Univ, Sch Food & Biotechnol, Key Lab Food & Biotechnol, Chengdu 610039, Peoples R China.;[Zhu, Longbao] Anhui Polytech Univ, Sch Biochem Engn, Wuhu 241000, Anhui, Peoples R China.
通讯机构：
[Sun, Weifeng] H;Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Peoples R China.
关键词：
Auto-inducible expression;Bacillus subtilis;Beefy meaty peptide;Fermentation production;Pontryagin's maximum principle
摘要：
Currently, some cases about the expression of flavor peptides with microorganisms were reported owing to the obvious advantages of biological expression over traditional methods. However, beefy meaty peptide (BMP), the focus of umami peptides, has neither been concerned in its safe expression nor its overproduction in fermenter. In this study, multi-copy BMP (8BMP) was successfully auto-inducibly expressed and efficiently produced in Bacillus subtilis 168. First, 8BMP was successfully auto-inducibly expressed with srfA promoter in B. subtilis 168. Further, the efficient production of 8BMP was researched in a 5-L fermenter: the fermentation optimized by Pontryagin's maximum principle obtained the highest 8BMP yield (3.16 g/L), which was 1.2 times and 1.8 times than that of two-stage feeding cultivation (2.67 g/L) and constant-rate feeding cultivation (1.75 g/L), respectively. Overall, the auto-inducible expression of 8BMP in B. subtilis and fermentation with Pontryagin's maximum principle are conductive for overproduction of BMP and other peptides.
摘要：
<jats:title>Summary</jats:title><jats:p>Preparation of flavour peptides with microorganisms is an attractive choice for its large‐scale production. However, beefy meaty peptide (BMP), as a research hotspot in the field of umami peptide, has been studied in few microorganisms, and furthermore, the safe preparation of BMP has not yet been reported. In this study, multi‐copy BMP (8BMP) was successfully expressed and produced in the ‘generally recognized as safe’ <jats:italic>Bacillus subtilis</jats:italic> (<jats:italic>B. subtilis</jats:italic>). Firstly, 8BMP with the potentially intense umami was screened out based on molecular docking analysis. Then, it was successfully expressed in the <jats:italic>B. subtilis</jats:italic> 168 and verified through sensory evaluation. Finally, the scaling capacity of 8BMP was evaluated in a 5‐L fermenter, with the highest yield (0.84gL<jats:sup>−1</jats:sup>) 6.4 times than that of shake flask, which is conductive for industrial production of BMP and other food peptides.</jats:p>
作者机构：
[Zhang, Wei; Peng, Daiyin; Xing, Shihai; Chen, Weidong; Cao, Mengyang] Anhui Univ Chinese Med, Coll Pharm, Room 405,Qianjiang Rd 1, Hefei 230012, Anhui, Peoples R China.;[Peng, Daiyin; Xing, Shihai; Cao, Mengyang] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;[Liu, Yingying] Anhui Univ Chinese Med, Coll Humanities & Int Educ Exchange, Hefei 230012, Peoples R China.;[Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Hunan, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, Minneapolis, MN 55108 USA.
通讯机构：
[Xing, Shihai] A;Anhui Univ Chinese Med, Coll Pharm, Room 405,Qianjiang Rd 1, Hefei 230012, Anhui, Peoples R China.;Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;Anhui Prov Key Lab Res & Dev Chinese Med, Hefei 230012, Peoples R China.
摘要：
<jats:title>Abstract</jats:title><jats:p><jats:italic>Salvia miltiorrhiza</jats:italic> has numerous compounds with extensive clinical application. “<jats:italic>Sweating</jats:italic>”, a processing method of Traditional Chinese Medicine (TCM), results in great changes in pharmacology and pharmacodynamics. Previously, chromatogram of 10 characteristic metabolites in <jats:italic>S</jats:italic>. <jats:italic>miltiorrhiza</jats:italic> showed a significant difference after “<jats:italic>Sweating</jats:italic>”. Due to the complexity of TCM, changes in metabolites should be investigated metabolome-wide after “<jats:italic>Sweating</jats:italic>”. An untargeted UPLC/MS-based metabolomics was performed to discover metabolites profile variation of <jats:italic>S</jats:italic>. <jats:italic>miltiorrhiza</jats:italic> after “<jats:italic>Sweating</jats:italic>”. Multivariate analysis was conducted to screen differential metabolites. Analysis indicated distinct differences between sweated and non-sweated samples. 10,108 substance peaks had been detected altogether, and 4759 metabolites had been identified from negative and positive ion model. 287 differential metabolites were screened including 112 up-regulated and 175 down-regulated and they belong to lipids and lipid-like molecules, and phenylpropanoid and polyketides. KEGG analysis showed the pathway of linoleic acid metabolism, and glyoxylate and dicarboxylate metabolism were mainly enriched. 31 and 49 identified metabolites were exclusively detected in SSM and NSSM, respectively, which mainly belong to carboxylic acids and derivatives, polyketides and fatty acyls. By mapping tanshinones and salvianolic acids to 4759 identified metabolites library, 23 characteristic metabolites had been identified, among which 11 metabolites changed most. We conclude that <jats:italic>“Sweating’’</jats:italic> has significant effect on metabolites content and composition of <jats:italic>S. miltiorrhiza</jats:italic>.</jats:p>
