作者机构：
[Zhang, Wei; Peng, Daiyin; Xing, Shihai; Chen, Weidong; Cao, Mengyang] Anhui Univ Chinese Med, Coll Pharm, Room 405,Qianjiang Rd 1, Hefei 230012, Anhui, Peoples R China.;[Peng, Daiyin; Xing, Shihai; Cao, Mengyang] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;[Liu, Yingying] Anhui Univ Chinese Med, Coll Humanities & Int Educ Exchange, Hefei 230012, Peoples R China.;[Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Hunan, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, Minneapolis, MN 55108 USA.
通讯机构：
[Xing, Shihai] A;Anhui Univ Chinese Med, Coll Pharm, Room 405,Qianjiang Rd 1, Hefei 230012, Anhui, Peoples R China.;Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;Anhui Prov Key Lab Res & Dev Chinese Med, Hefei 230012, Peoples R China.
摘要：
Toxoplasma gondii (T. gondii) is an important pathogen that can cause serious public health problems. Currently, therapeutic drugs for toxoplasmosis present serious side effects, researches on more effective and novel substances with relatively low toxicity are urgently needed. Spider venoms comprise diverse novel pharmacological compounds. However, the anti-T. gondii activity of spider venoms remains largely unknown. This study was carried out to evaluate the anti-parasitic effect of spider venoms from Ornitoctonus huwena (HWVM) and Chilobrachys jingzhao (JZVM) against T. gondii tachyzoites in vitro and in vivo. Cytotoxic activity of HWVM and JZVM to HeLa cells was determined by MTT cell viability assays. Low doses (3.125, 6.25 and 12.5mug/mL) of HWVM and JZVM displayed low toxicity to HeLa cells. Trypan blue exclusion assay indicated that either of HWVM and JZVM affected the viability of tachyzoites in a time-dependent manner. Both spider venoms inhibited the invasion and proliferation of tachyzoites in vitro (p<0.05). Moreover, Mice treated with HWVM after infection with 2x10(3)T. gondii tachyzoites showed a better survival rate than mice treated with saline alone (p<0.05), while mice treated with JZVM did not. Our findings indicate that HWVM is a promising agent for the treatment of toxoplasmosis.
摘要：
Toxoplasmosis is a widely distributed parasitic protozoan disease, caused by Toxoplasma gondii (T. gondii). High prevalence of toxoplasmosis and limitations of conventional treatments lead to a search for new therapeutic drugs. Lycosin-I is a linear peptide, derived from the venom of the spider Lycosa singoriensis. The aim of the present study was to determine the anti-parasitic effect of lycosin-Iota against T. gondii. In vitro, the anti-T. gondii activities of lycosin-Iota were evaluated by MTT assay, trypan blue exclusion assay, cell counting assay and plaque assay. Cytokines of IL-6 and IL-8 were measured by quantitative PCR. In addition, the structures of tachyzoites treated with lycosin-Iota were also observed by scanning and transmission electron microscopy. In vivo, mice were challenged with parasites treated by lycosin-I. The results revealed that lycosin-Iota had shown a significant ability to inhibit T. gondii invasion and proliferation. Cytokines of IL-6 and IL-8 were reduced by lycosin-Iota at transcription level in human foreskin fibroblast (HFF) cells infected with T. gondii tachyzoites, but they were increased compared to non-infected cells. For tachyzoites, lycosin-Iota induced their cell membrane alterations with formation of invaginations, some of them appeared to be vacuolated in their cytoplasm. Moreover, lycosin-Iota had prolonged the survival time of mice by controlling T. gondii proliferation. In conclusion, our present study provides the first evidence for anti-T. gondii by using the spider peptide lycosin-Iota. These findings suggest that lycosin-Iota is a potential alternative agent for the treatment of toxoplasmosis.
摘要：
In many-objective optimization, the balance between convergence and diversity is hard to maintain, while the dominance resistant solutions (DRSs) could further harm the balance particularly in high-dimensional objective space. Thus, this paper proposes a novel selection strategy - boundary elimination selection based on binary search (called BESBS), trying to avoid the impact of DRSs during the optimization and achieve a good balance between the convergence and diversity simultaneously. During the environmental selection, the binary search (BS) is used to adaptively adjust the epsilon value in the epsilon-dominance relationship and assist in detecting the well-distributed neighbors for the elite solutions. Then the epsilon value obtained by BS is used for serving the boundary elimination selection (BES) to guarantee the stability of the elite population. To improve the convergence, BES is mainly designed to select individuals approximating to the ideal point. By modifying the fitness of solutions and choosing solutions in terms of the shuffled sequence of objective axis, the DRSs will be eliminated during the selection. Thus, BESBS could achieve a good balance between the convergence and diversity and avoid the impact from DRSs simultaneously. From a series of experiments with 35 instances, the experimental results have shown that BESBS is competitive against 8 state-of-art many-objective evolutionary algorithms. (C) 2017 Elsevier B.V. All rights reserved.
摘要：
Schiff base organotin(IV) complexes C1 similar to C5b have been synthesized via the reaction of the substituted salicylaldehyde-o-aminophenol Schiff base ligands (L1 similar to L3) with the dibenzyltin dichloride, n-butyltin trichloride or dibutyltin oxide, respectively. The complexes have been characterized by IR, UV Vis, 1H NMR, 13C NMR spectra, elemental analysis and the crystal structures have been determined by X-ray diffraction. The anticancer activity of the Schiff base ligand and complexes C1 similar to C5b against five species of cancer cell which are Hela, MCF7, HepG2, Colo205, NCI-H460 were tested respectively, the tests showed that C1 similar to C5b exhibited significant anticancer activity for the cancer cells in comparison with the ligand, and the activity was greater than carboplatin. (C) 2017 Published by Elsevier B.V.
作者：
Fu, W. W.*;Cheng, Q. W.;Wang, H. Y.;He, F. Z. R.;Liu, Y.;...
期刊：
Russian Journal of Coordination Chemistry,2017年43(8):547-558 ISSN：1070-3284
通讯作者：
Fu, W. W.
作者机构：
[Zhang, F. X.; Fu, W. W.; Cheng, Q. W.; He, F. Z. R.; Liu, Y.; Wang, H. Y.] Hengyang Normal Univ, Dept Chem & Mat Sci, Hunan Prov Coll, Key Lab Funct Organometall Mat, Hengyang 421008, Peoples R China.
通讯机构：
[Fu, W. W.] H;Hengyang Normal Univ, Dept Chem & Mat Sci, Hunan Prov Coll, Key Lab Funct Organometall Mat, Hengyang 421008, Peoples R China.
摘要：
Four homoleptic copper(II) complexes, [Cu(Meophtpy)2](ClO4)2 (Meophtpy = 4′-(4-methoxylphenyl)- 2,2′:6′,2″-terpyridine) (I), [Cu(Meophtpy)2](ClO4)2 · 2H2O (II), [Cu2(m-Clphtpy)4](ClO4)4 (m-ClPhtpy = 4′-(3-chlorophenyl)-2,2′:6′,2″-terpyridine) (III), and [Cu2(m-ClPhtpy)4](ClO4)4 (IV) have been synthesized by hydrothermal methods and characterized by IR, elemental analysis and single crystal X-ray diffraction (CIF files CCDC nos. 963375 (I), 885457 (II), 963377 (III), and 963376 (IV)). Complex II is a polymorph of I and complex IV is a polymorph of III. All these complexes are obtained with 95% ethanol solution or 50% ethanol solution and the solvent control on the crystallization are obviously found. In all complexes, the face-to-face interactions between pyridyl rings or phenyl rings facilitate the construction of 3D network in the crystal in addition to hydrogen bonds. The fluorescence properties of these complexes have been investigated.
摘要：
In this paper, we focus on the problem of similarity assessment of isometric 3D shapes, which is of great relevance in improving the effectiveness of retrieval tasks. We first present an effective shape representation technique by proposing a partial aggregation model based on the bag-of-words paradigm. This technique can effectively encode our multiscale local features and has a good discriminatory ability. We then develop a parameter-free distance mapping approach to re-evaluate the similarity results based on intrinsic analysis of a well organized reciprocal k-nearest neighborhood graph. Different from the existing methods which determine k manually and globally, the proposed method can automatically adjust k to a reliable local domain, which therefore ensures a more accurate similarity measurement. We fully study our shape representation technique and evaluate the performance of the proposed distance mapping approach on several popular public shape benchmarks. Experiment results have demonstrated the state-of-the-art performance of our approach. (C) 2016 Elsevier B.V. Allrightsreserved.
摘要：
Two coordination complexes [Cu(phtpy)(obda)]center dot 2H(2)O (1) and [Cu-4(meophtpy)(4)(mbda)(4)]center dot 4H(2)O (2) [phtpy = 4'-phenyl-2,2':6',2aEuro(3)-terpyridine, meophtpy = 4'-(4-methoxyphenyl)-2,2':6',2aEuro(3)-terpyridine, H(2)obda = 1,2-benzenedicarboxylic acid and H(2)mbda = 1,3-benzenedicarboxylic acid] have been synthesized under hydrothermal conditions and characterized by IR, elemental analysis and X-ray single-crystal diffraction. In complex 1, the Cu(II) atoms are bridged by obda(2-) ligands to give 1D chains with phtpy as a terminal ligand, while a 3D network is constructed by hydrogen bonds and pi-pi interactions. In complex 2, the Cu(II) atoms are coordinated by two meophtpy ligands and also linked by mbda(2-) ligands forming a discrete tetranuclear complex. In this complex also, a 3D network is constructed by hydrogen bonds and pi-pi interactions. The most striking feature of complex 2 is that one meophtpy ligand is almost overlapped upon the next which is seldom found in 2,2':6',2aEuro(3)-terpyridine complexes. The luminescence properties of both complexes 1 and 2 have been investigated.
期刊：
European Archives of Oto-Rhino-Laryngology,2015年272(11):3299-3299 ISSN：0937-4477
通讯作者：
Xiao, Bing
作者机构：
[Tu, Menjiang; Ai, Kai] Cent S Univ, Dept Urol, Xiangya Hosp 2, Changsha, Hunan, Peoples R China.;[Sun, Mai] Cent S Univ, Dept Otolaryngol, Xiangya Sch Med, Changsha, Hunan, Peoples R China.;[Liu, Yonggang] Hengyang Normal Coll, Dept Sports Med, Hengyang, Peoples R China.;[Guo, Bowen] Cent S Univ, Xiangya Sch Med, Changsha, Hunan, Peoples R China.;[Zhao, Jiapeng] Cent S Univ, Dept Neurosurg, Xiangya Sch Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Xiao, Bing] C;Cent S Univ, Dept Otolaryngol, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China.
摘要：
To investigate the role of total facial nerve decompression in severe idiopathic recurrent facial palsy. A consecutive series of 22 cases with severe idiopathic recurrent facial palsy were managed in our hospital between January, 2003 and January, 2011. Either total facial nerve decompression or conservative treatment was provided, and they were divided into surgery group and control group according to their choice of treatment. They were followed up for at least 3 years. Facial palsy recurrence and facial nerve outcomes in the two groups were compared. There were 12 patients in surgery group, who were followed up for 5.3 years (range 3-8 years). In contrast, there were 10 patients in control group, with an average follow-up of 5.2 years (range 3-7 years). None of the patients (0 %) in surgery group recurred compared to 4 of 10 cases (40 %) in control group (p < 0.05). In perspectives of facial nerve outcomes, 11 of 12 cases (91.7 %) in surgery group achieved good recovery (HB Grade I or II) in contrast to 3 of 10 cases (30.0 %) in control group (p < 0.05). For severe idiopathic recurrent facial palsy, total facial nerve decompression could not only prevent further recurrence but also promote facial nerve recovery.
