期刊：
Bioprocess and Biosystems Engineering,2020年43(4):701-710 ISSN：1615-7591
通讯作者：
Sun, Weifeng
作者机构：
[Chen, Xiaohua; Sun, Weifeng; Liu, Yi] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Peoples R China.;[Wang, Li; Lin, Lu; Wu, Lunjie; Ding, Wenwu; Che, Zhenming; Wu, Yuanming] Xihua Univ, Sch Food & Biotechnol, Key Lab Food & Biotechnol, Chengdu 610039, Peoples R China.;[Zhu, Longbao] Anhui Polytech Univ, Sch Biochem Engn, Wuhu 241000, Anhui, Peoples R China.
通讯机构：
[Sun, Weifeng] H;Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Peoples R China.
关键词：
Auto-inducible expression;Bacillus subtilis;Beefy meaty peptide;Fermentation production;Pontryagin's maximum principle
摘要：
Currently, some cases about the expression of flavor peptides with microorganisms were reported owing to the obvious advantages of biological expression over traditional methods. However, beefy meaty peptide (BMP), the focus of umami peptides, has neither been concerned in its safe expression nor its overproduction in fermenter. In this study, multi-copy BMP (8BMP) was successfully auto-inducibly expressed and efficiently produced in Bacillus subtilis 168. First, 8BMP was successfully auto-inducibly expressed with srfA promoter in B. subtilis 168. Further, the efficient production of 8BMP was researched in a 5-L fermenter: the fermentation optimized by Pontryagin's maximum principle obtained the highest 8BMP yield (3.16 g/L), which was 1.2 times and 1.8 times than that of two-stage feeding cultivation (2.67 g/L) and constant-rate feeding cultivation (1.75 g/L), respectively. Overall, the auto-inducible expression of 8BMP in B. subtilis and fermentation with Pontryagin's maximum principle are conductive for overproduction of BMP and other peptides.
作者机构：
[Zhang, Wei; Peng, Daiyin; Xing, Shihai; Chen, Weidong; Cao, Mengyang] Anhui Univ Chinese Med, Coll Pharm, Room 405,Qianjiang Rd 1, Hefei 230012, Anhui, Peoples R China.;[Peng, Daiyin; Xing, Shihai; Cao, Mengyang] Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;[Liu, Yingying] Anhui Univ Chinese Med, Coll Humanities & Int Educ Exchange, Hefei 230012, Peoples R China.;[Jiang, Weimin] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Hunan, Peoples R China.;[Meng, Xiaoxi] Univ Minnesota, Dept Hort Sci, Minneapolis, MN 55108 USA.
通讯机构：
[Xing, Shihai] A;Anhui Univ Chinese Med, Coll Pharm, Room 405,Qianjiang Rd 1, Hefei 230012, Anhui, Peoples R China.;Anhui Acad Chinese Med, Inst Tradit Chinese Med Resources Protect & Dev, Hefei 230012, Peoples R China.;Anhui Prov Key Lab Res & Dev Chinese Med, Hefei 230012, Peoples R China.
摘要：
<jats:title>Abstract</jats:title><jats:p><jats:italic>Salvia miltiorrhiza</jats:italic> has numerous compounds with extensive clinical application. “<jats:italic>Sweating</jats:italic>”, a processing method of Traditional Chinese Medicine (TCM), results in great changes in pharmacology and pharmacodynamics. Previously, chromatogram of 10 characteristic metabolites in <jats:italic>S</jats:italic>. <jats:italic>miltiorrhiza</jats:italic> showed a significant difference after “<jats:italic>Sweating</jats:italic>”. Due to the complexity of TCM, changes in metabolites should be investigated metabolome-wide after “<jats:italic>Sweating</jats:italic>”. An untargeted UPLC/MS-based metabolomics was performed to discover metabolites profile variation of <jats:italic>S</jats:italic>. <jats:italic>miltiorrhiza</jats:italic> after “<jats:italic>Sweating</jats:italic>”. Multivariate analysis was conducted to screen differential metabolites. Analysis indicated distinct differences between sweated and non-sweated samples. 10,108 substance peaks had been detected altogether, and 4759 metabolites had been identified from negative and positive ion model. 287 differential metabolites were screened including 112 up-regulated and 175 down-regulated and they belong to lipids and lipid-like molecules, and phenylpropanoid and polyketides. KEGG analysis showed the pathway of linoleic acid metabolism, and glyoxylate and dicarboxylate metabolism were mainly enriched. 31 and 49 identified metabolites were exclusively detected in SSM and NSSM, respectively, which mainly belong to carboxylic acids and derivatives, polyketides and fatty acyls. By mapping tanshinones and salvianolic acids to 4759 identified metabolites library, 23 characteristic metabolites had been identified, among which 11 metabolites changed most. We conclude that <jats:italic>“Sweating’’</jats:italic> has significant effect on metabolites content and composition of <jats:italic>S. miltiorrhiza</jats:italic>.</jats:p>
通讯机构：
[Liu, Y; Lei, W] C;[Liu, Y; Lei, W] X;Changsha Univ, Coll Comp Engn & Appl Math, Changsha 410022, Peoples R China.;Xiangtan Univ, Key Lab Hunan Prov Internet Things & Informat Sec, Xiangtan 411105, Peoples R China.
关键词：
Proteins;Predictive models;Databases;Computational modeling;Prediction algorithms;Biological system modeling;Essential protein;PPI;SimRank algorithm;PageRank algorithm
摘要：
Identification of essential proteins is important for understanding cell survival and development, because even if only one of these proteins is missing, organisms cannot survive or develop. Since traditional methods for identifying essential proteins based on biological experiments are costly and inefficient, more and more computational models are proposed for predicting essential proteins in recent years. In this paper, a novel computational model called BSPM is proposed, in which, an original PPI network will be built based on known protein-protein associations first, and then topology information of the original PPI network will be adopted to measure the similarities between proteins based on the SimRank algorithm. Thereafter, a weighted PPI network can be obtained based on the similarities between proteins and the original PPI network. Finally, based on the weighted PPI network, the PageRank algorithm will be used to infer potential essential proteins. Moreover, in order to evaluate the performance of BSPM, we have compared the performance of BSPM with 14 classical prediction models in the field based on two different databases, and experimental results show that BSPM can achieve prediction accuracies of 92%, 81% and 76% out of the top 100, 200 and 300 candidate proteins separately, which not only are significantly better than those 14 competitive classical prediction models, but also means that BSPM can be used as an effective model for identifying essential proteins in the future.
摘要：
Toxoplasma gondii (T. gondii) is an important pathogen that can cause serious public health problems. Currently, therapeutic drugs for toxoplasmosis present serious side effects, researches on more effective and novel substances with relatively low toxicity are urgently needed. Spider venoms comprise diverse novel pharmacological compounds. However, the anti-T. gondii activity of spider venoms remains largely unknown. This study was carried out to evaluate the anti-parasitic effect of spider venoms from Ornitoctonus huwena (HWVM) and Chilobrachys jingzhao (JZVM) against T. gondii tachyzoites in vitro and in vivo. Cytotoxic activity of HWVM and JZVM to HeLa cells was determined by MTT cell viability assays. Low doses (3.125, 6.25 and 12.5mug/mL) of HWVM and JZVM displayed low toxicity to HeLa cells. Trypan blue exclusion assay indicated that either of HWVM and JZVM affected the viability of tachyzoites in a time-dependent manner. Both spider venoms inhibited the invasion and proliferation of tachyzoites in vitro (p<0.05). Moreover, Mice treated with HWVM after infection with 2x10(3)T. gondii tachyzoites showed a better survival rate than mice treated with saline alone (p<0.05), while mice treated with JZVM did not. Our findings indicate that HWVM is a promising agent for the treatment of toxoplasmosis.
摘要：
Toxoplasmosis is a widely distributed parasitic protozoan disease, caused by Toxoplasma gondii (T. gondii). High prevalence of toxoplasmosis and limitations of conventional treatments lead to a search for new therapeutic drugs. Lycosin-I is a linear peptide, derived from the venom of the spider Lycosa singoriensis. The aim of the present study was to determine the anti-parasitic effect of lycosin-Iota against T. gondii. In vitro, the anti-T. gondii activities of lycosin-Iota were evaluated by MTT assay, trypan blue exclusion assay, cell counting assay and plaque assay. Cytokines of IL-6 and IL-8 were measured by quantitative PCR. In addition, the structures of tachyzoites treated with lycosin-Iota were also observed by scanning and transmission electron microscopy. In vivo, mice were challenged with parasites treated by lycosin-I. The results revealed that lycosin-Iota had shown a significant ability to inhibit T. gondii invasion and proliferation. Cytokines of IL-6 and IL-8 were reduced by lycosin-Iota at transcription level in human foreskin fibroblast (HFF) cells infected with T. gondii tachyzoites, but they were increased compared to non-infected cells. For tachyzoites, lycosin-Iota induced their cell membrane alterations with formation of invaginations, some of them appeared to be vacuolated in their cytoplasm. Moreover, lycosin-Iota had prolonged the survival time of mice by controlling T. gondii proliferation. In conclusion, our present study provides the first evidence for anti-T. gondii by using the spider peptide lycosin-Iota. These findings suggest that lycosin-Iota is a potential alternative agent for the treatment of toxoplasmosis.
摘要：
<jats:title>Abstract</jats:title><jats:p>The electron‐transporting material (ETM) is one of the key factors to determine the efficiency and stability of organic light‐emitting diodes (OLEDs). A novel ETM with a “(Acceptor)<jats:sub>n</jats:sub>–Donor–(Acceptor)<jats:sub>n</jats:sub>” (“(A)<jats:sub>n</jats:sub>–D–(A)<jats:sub>n</jats:sub>”) structure, 2,7‐di([2,2′:6′,2″‐terpyridin]‐4′‐yl)‐9,9′‐spirobifluorene (27‐TPSF), is synthesized by combining electron‐withdrawing terpyridine (TPY) moieties and rigid twisted spirobifluorene, in which the TPY moieties facilitate electron transport and injection while the spirobifluorene moiety ensures high triplet energy (<jats:italic>T</jats:italic><jats:sub>1</jats:sub> = 2.5 eV) as well as enhances glass transition temperature (<jats:italic>T</jats:italic><jats:sub>g</jats:sub> = 195 °C) for better stability. By using tris[2‐(<jats:italic>p‐</jats:italic>tolyl)pyridine]iridium(III) (Ir(mppy)<jats:sub>3</jats:sub>) as the emitter, the 27‐TPSF‐based device exhibits a maximum external quantum efficiency (η<jats:sub>ext, max</jats:sub>) of 24.5%, and a half‐life (T<jats:sub>50</jats:sub>) of 121, 6804, and 382 636 h at an initial luminance of 10 000, 1000, and 100 cd m<jats:sup>−2</jats:sup>, respectively, which are much better than the commercialized ETM of 9,10‐bis(6‐phenylpyridin‐3‐yl)anthracene (DPPyA). Furthermore, a higher efficiency, a η<jats:sub>ext, max</jats:sub> of 28.2% and a maximum power efficiency (η<jats:sub>PE</jats:sub>, <jats:sub>max</jats:sub>) of 129.3 lm W<jats:sup>−1</jats:sup>, can be achieved by adopting bis(2‐phenylpyridine)iridium(III)(2,2,6,6‐tetramethylheptane‐3,5‐diketonate) (Ir(ppy)<jats:sub>2</jats:sub>tmd) as the emitter and 27‐TPSF as the ETM. These results indicate that the derivative of TPY to form “(A)<jats:sub>n</jats:sub>–D–(A)<jats:sub>n</jats:sub>” structure is a promising way to design an ETM with good comprehensive properties for OLEDs.</jats:p>
摘要：
In many-objective optimization, the balance between convergence and diversity is hard to maintain, while the dominance resistant solutions (DRSs) could further harm the balance particularly in high-dimensional objective space. Thus, this paper proposes a novel selection strategy - boundary elimination selection based on binary search (called BESBS), trying to avoid the impact of DRSs during the optimization and achieve a good balance between the convergence and diversity simultaneously. During the environmental selection, the binary search (BS) is used to adaptively adjust the epsilon value in the epsilon-dominance relationship and assist in detecting the well-distributed neighbors for the elite solutions. Then the epsilon value obtained by BS is used for serving the boundary elimination selection (BES) to guarantee the stability of the elite population. To improve the convergence, BES is mainly designed to select individuals approximating to the ideal point. By modifying the fitness of solutions and choosing solutions in terms of the shuffled sequence of objective axis, the DRSs will be eliminated during the selection. Thus, BESBS could achieve a good balance between the convergence and diversity and avoid the impact from DRSs simultaneously. From a series of experiments with 35 instances, the experimental results have shown that BESBS is competitive against 8 state-of-art many-objective evolutionary algorithms. (C) 2017 Elsevier B.V. All rights reserved.
摘要：
The polarization evolution of vector beams (VBs) generated by q-plates is investigated theoretically and experimentally. An analytical model is developed for the VB created by a general quarter-wave q-plate based on vector diffraction theory. It is found that the polarization distribution of VBs varies with position and the value q. In particular, for the incidence of circular polarization, the exit vector vortex beam has polarization states that cover the whole surface of the Poincare sphere, thereby constituting a full Poincare beam. For the incidence of linear polarization, the VB is not cylindrical but specularly symmetric, and exhibits an azimuthal spin splitting. These results are in sharp contrast with those derived by the commonly used model, i.e., regarding the incident light as a plane wave. By implementing q-plates with dielectric metasurfaces, further experiments validate the theoretical results. (C) 2017 Chinese Laser Press
作者：
Fu, W. W.*;Cheng, Q. W.;Wang, H. Y.;He, F. Z. R.;Liu, Y.;...
期刊：
Russian Journal of Coordination Chemistry,2017年43(8):547-558 ISSN：1070-3284
通讯作者：
Fu, W. W.
作者机构：
[Zhang, F. X.; Fu, W. W.; Cheng, Q. W.; He, F. Z. R.; Liu, Y.; Wang, H. Y.] Hengyang Normal Univ, Dept Chem & Mat Sci, Hunan Prov Coll, Key Lab Funct Organometall Mat, Hengyang 421008, Peoples R China.
通讯机构：
[Fu, W. W.] H;Hengyang Normal Univ, Dept Chem & Mat Sci, Hunan Prov Coll, Key Lab Funct Organometall Mat, Hengyang 421008, Peoples R China.
摘要：
Four homoleptic copper(II) complexes, [Cu(Meophtpy)2](ClO4)2 (Meophtpy = 4′-(4-methoxylphenyl)- 2,2′:6′,2″-terpyridine) (I), [Cu(Meophtpy)2](ClO4)2 · 2H2O (II), [Cu2(m-Clphtpy)4](ClO4)4 (m-ClPhtpy = 4′-(3-chlorophenyl)-2,2′:6′,2″-terpyridine) (III), and [Cu2(m-ClPhtpy)4](ClO4)4 (IV) have been synthesized by hydrothermal methods and characterized by IR, elemental analysis and single crystal X-ray diffraction (CIF files CCDC nos. 963375 (I), 885457 (II), 963377 (III), and 963376 (IV)). Complex II is a polymorph of I and complex IV is a polymorph of III. All these complexes are obtained with 95% ethanol solution or 50% ethanol solution and the solvent control on the crystallization are obviously found. In all complexes, the face-to-face interactions between pyridyl rings or phenyl rings facilitate the construction of 3D network in the crystal in addition to hydrogen bonds. The fluorescence properties of these complexes have been investigated.
