期刊：
Journal of Food Measurement and Characterization,2023年17(3):2386-2403 ISSN：2193-4126
通讯作者：
Lou, Zaixiang(louzaixiang@126.com)
作者机构：
[Zheng, Xiaodan; Dai, Wenna; Bede, Dery; Lou, Zaixiang; Wang, Hongxin] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China.;[Zheng, Xiaodan; Dai, Wenna; Bede, Dery; Lou, Zaixiang; Wang, Hongxin] Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Peoples R China.;[Wan, Chunpeng] Jiangxi Agr Univ, Coll Agron, Collaborat Innovat Ctr Post Harvest Key Technol &, Jiangxi Key Lab Post Harvest Technol & Nondestruct, Nanchang 330045, Peoples R China.;[Chen, Xiaohua] Hengyang Normal Univ, Hengyang 421001, Peoples R China.
通讯机构：
[Zaixiang Lou] S;State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of China<&wdkj&>National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, People’s Republic of China
摘要：
Biofilm is a complex community of microorganisms with a more stable form compared to planktonic bacteria. Probiotic strains in biofilms have been found to have beneficial effects in numerous studies. The formation process and the regulatory mechanisms of biofilm were introduced. Strategies to promote biofilm growth were reviewed in categories. And the extraction and purification process of natural products in exogenous substances was also sorted out and summarized. The research found that different modes of action of the promoting biofilm molecules discussed were facilitated by interfering with the quorum sensing pathways, adhesion mechanisms, the two-component system, influencing extracellular polymers, and secondary messengers involved in various signaling pathways. The studies showed that the strength of the biofilm can be promoted by altering the adhesion surface material and nutrient sources. Probiotics and their secondary metabolites made critical impacts. Further studies found that the ingredients that can promote the formation of bacterial biofilms include hormones, inorganic ions (Mn2+, Cu2+, Fe3+), and natural extracts (inulin, fructooligosaccharides, malic acid, catechins, genistein, cranberry extract, resveratrol, propolis ethanolic extract, bioactive peptides, etc.). The method of extraction and purification prefers safe and low-harm solvents such as ethanol and water. Physical methods were also used to improve yields, such as microwaves, ultra-high pressure, column chromatography, etc. A multi-point, staggered, and all-round examination of the development of probiotic biofilms was directed. The exploration of probiotic biofilm will be a significant advancement heading in the field of probiotic research.
作者机构：
[Zhang Hao; Chen Wei; Wang Gang; He Yufeng; Zhao Jianxin; Jin Xing] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China.;[Zhang Hao; Chen Wei; Wang Gang; He Yufeng; Zhao Jianxin; Jin Xing] Jiangnan Univ, Schl Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China.;[Zhou Yonghua] Jiangsu Inst Parasit Dis, Jiangsu Prov Key Lab Parasite & Vector Control Te, Key Lab Natl Hlth & Family Plann Commis, Parasit Dis Control & Prevent, Wuxi 214064, Jiangsu, Peoples R China.;[Chen Xiaohua] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Peoples R China.;[Lee Yuan-Kun] Natl Univ Singapore, Dept Microbiol & Immunol Natl, Singapore 117597, Singapore.
通讯机构：
[Wang, Gang] S;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.;School of Food Science and Technology, Jiangnan University, Wuxi, 214122, P. R. China.;International Joint Research Laboratory for Probiotics, Jiangnan University, Wuxi, 214122, P. R. China.;(Yangzhou) Institute of Food Biotechnology, Jiangnan University, Yangzhou, 225004, P. R. China.
摘要：
Campylobacter jejuni is the major micro-bacillary pathogen responsible for human coloenteritis. Lactic acid bacteria (LAB) have been shown to protect against Campylobacter infection. However, LAB with a good ability to inhibit the growth of C. jejuni in vitro are less effective in animals and animal models, and have the disadvantages of high cost, a long cycle, cumbersome operation and insignificant immune response indicators. Caenorhabditis elegans is increasingly used to screen probiotics for their anti-pathogenic properties. However, no research on the use of C. elegans to screen for probiotic candidates antagonistic to C. jejuni has been conducted to date. This study established a lifespan model of C. elegans, enabling the preselection of LAB to counter C. jejuni infection. A potential protective mechanism of LAB was identified. Some distinct LAB species offered a high level of protection to C. elegans against C. jejuni. The LAB strains with a high protection rate reduced the load of C. jejuni in C. elegans. The transcription of antibacterial peptide genes, MAPK and Daf-16 signalling pathway-related genes was elevated using the LAB isolates with a high protection rate. The reliability of the lifespan model of C. elegans was verified using mice and chickens infected with C. jejuni. The results showed that different LAB had different abilities to protect C. elegans against C. jejuni. C. elegans provides a reliable model for researchers to screen for LAB that are antagonistic to C. jejuni on a large scale.
期刊：
Bioprocess and Biosystems Engineering,2020年43(4):701-710 ISSN：1615-7591
通讯作者：
Sun, Weifeng
作者机构：
[Chen, Xiaohua; Sun, Weifeng; Liu, Yi] Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Peoples R China.;[Wang, Li; Lin, Lu; Wu, Lunjie; Ding, Wenwu; Che, Zhenming; Wu, Yuanming] Xihua Univ, Sch Food & Biotechnol, Key Lab Food & Biotechnol, Chengdu 610039, Peoples R China.;[Zhu, Longbao] Anhui Polytech Univ, Sch Biochem Engn, Wuhu 241000, Anhui, Peoples R China.
通讯机构：
[Sun, Weifeng] H;Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Peoples R China.
关键词：
Auto-inducible expression;Bacillus subtilis;Beefy meaty peptide;Fermentation production;Pontryagin's maximum principle
摘要：
Currently, some cases about the expression of flavor peptides with microorganisms were reported owing to the obvious advantages of biological expression over traditional methods. However, beefy meaty peptide (BMP), the focus of umami peptides, has neither been concerned in its safe expression nor its overproduction in fermenter. In this study, multi-copy BMP (8BMP) was successfully auto-inducibly expressed and efficiently produced in Bacillus subtilis 168. First, 8BMP was successfully auto-inducibly expressed with srfA promoter in B. subtilis 168. Further, the efficient production of 8BMP was researched in a 5-L fermenter: the fermentation optimized by Pontryagin's maximum principle obtained the highest 8BMP yield (3.16 g/L), which was 1.2 times and 1.8 times than that of two-stage feeding cultivation (2.67 g/L) and constant-rate feeding cultivation (1.75 g/L), respectively. Overall, the auto-inducible expression of 8BMP in B. subtilis and fermentation with Pontryagin's maximum principle are conductive for overproduction of BMP and other peptides.
作者机构：
[郭敏; 姜杨; 赵建新] School of Food Science and Technology, Jiangnan University, Wuxi;214122, China;[王承瑞; 陈晓华] College of Life Sciences and Environment, Hengyang Normal University, Hengyang;421008, China;Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, Hengyang
作者机构：
[倪彩新; 张灏; 金星; 陈卫; Zhang H.; 王刚] School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China;[周炜; 印伯星; 房东升] Kangyuan Dairy Co., Ltd., Yangzhou University, Yangzhou, 225004, China;[陈晓华] College of Life Sciences and Environment, Hengyang Normal University, Hengyang, 421008, China
通讯机构：
[Wang, G.] S;School of Food Science and Technology, China
